Waley S G
Biochem J. 1974 Jun;139(3):789-90. doi: 10.1042/bj1390789.
A new method for measuring the enzymic hydrolysis of the beta-lactam ring in penicillins is described. The change in extinction in the u.v. region is determined. The method is sensitive (50mum-benzylpenicillin can be used) and convenient.
本文描述了一种测定青霉素中β-内酰胺环酶促水解的新方法。测定了紫外区域吸光度的变化。该方法灵敏(可用50μm的苄青霉素)且简便。