Kimura Y, Inoue Y, Honda H
Immunology. 1974 May;26(5):983-8.
With a modified rat mast cell degranulation (RMCD) technique developed by Korotzer, Haddad and Lopapa (1971), the mechanism of mast cell degranulation by IgE—anti-IgE reaction and the inhibitory effect of cAMP-related compounds upon IgE-mediated mast cell degranulation were studied. Degranulations of 90 per cent or more were decreased to 13–16 per cent when the mast cells were pretreated with human IgE or normal human serum. However, if rat mast cells were pretreated with anti-human IgE rabbit serum or normal rabbit serum, the degranulation per cent in these cells by IgE—anti-IgE reaction was the same as in the nontreated cells. These results suggest the presence of receptors in rat mast cells for human IgE or normal human serum, and the lack of receptors in these cells for anti-human IgE rabbit serum or normal rabbit serum. Treatment of isolated rat mast cells with adenyl cyclase stimulating agents (isoprenaline, adrenaline, prostaglandin E and E) and theophylline or aminophylline, which inhibit the enzymatic degradation of cAMP, also inhibited the morphological degranulation of the mast cells. Cromoglycate or chlorophenes in derivatives, which might have a stabilizing effect of the cell membrane, also inhibited the degranulation of the rat mast cells mediated by IgE—anti-IgE reaction. These results support the attractive hypothesis that cAMP occupies a central modulatory role in the mast cell degranulation by IgE—anti-IgE reaction.
采用由科罗策、哈达德和洛帕帕（1971年）研发的改良大鼠肥大细胞脱颗粒（RMCD）技术，研究了IgE - 抗IgE反应引起肥大细胞脱颗粒的机制以及环磷酸腺苷（cAMP）相关化合物对IgE介导的肥大细胞脱颗粒的抑制作用。当肥大细胞用人IgE或正常人血清预处理时，90%或更高比例的脱颗粒现象减少至13% - 16%。然而，如果大鼠肥大细胞用抗人IgE兔血清或正常兔血清预处理，这些细胞通过IgE - 抗IgE反应的脱颗粒百分比与未处理细胞相同。这些结果表明大鼠肥大细胞中存在人IgE或正常人血清的受体，而这些细胞中不存在抗人IgE兔血清或正常兔血清的受体。用腺苷酸环化酶刺激剂（异丙肾上腺素、肾上腺素、前列腺素E和E）以及抑制cAMP酶促降解的茶碱或氨茶碱处理分离的大鼠肥大细胞，也抑制了肥大细胞的形态学脱颗粒。可能对细胞膜有稳定作用的色甘酸或氯苯那敏衍生物也抑制了由IgE - 抗IgE反应介导的大鼠肥大细胞脱颗粒。这些结果支持了一个有吸引力的假说，即cAMP在IgE - 抗IgE反应引起的肥大细胞脱颗粒中起核心调节作用。
