Glaser E M
Exp Brain Res. 1979 Mar 9;35(1):193-5. doi: 10.1007/BF00236794.
A small, movable aperture placed at or near the Köhler field diaphragm location of a light microscope permits making composite high contrast, high clarity photographs of objects, such as Golgi stained neurons, whose extensions in depth are greater than the depth of field of the microscope's objective. The technique is described.
在光学显微镜的柯勒场光阑位置或其附近放置一个小型可移动孔径,能够拍摄如高尔基染色神经元等物体的合成高对比度、高清晰度照片,这些物体在深度上的延伸大于显微镜物镜的景深。本文描述了该技术。