Freire M, Boyde A
Instituto Cajal, C.S.I.C., Madrid, Spain.
J Neurosci Methods. 1995 May;58(1-2):109-16. doi: 10.1016/0165-0270(94)00165-d.
A correlative study of neuronal reconstruction methods was made using both conventional (non-confocal) and real-time confocal microscopies. Simple and sophisticated (totally automated) methods are described for making both biplanar microphotographs using conventional transmitted light, and stereoscopic microphotographs using real-time confocal microscopy of Golgi-impregnated neurons. Confocal microscopy discriminates against out-of-focus layers to produce optical sections which can be summed on photographic film to obtain neuronal reconstructions. Biplanar images are obtained by fusion, using a stereo-viewer, of two adjacent optical sections obtained with a conventional transmitted light microscope. Stereoscopic and biplanar microphotographs of 3-day-old chick neurons are presented.
利用传统（非共聚焦）显微镜和实时共聚焦显微镜对神经元重建方法进行了相关性研究。描述了简单和复杂（完全自动化）的方法，用于使用传统透射光制作双平面显微照片，以及使用高尔基体浸染神经元的实时共聚焦显微镜制作立体显微照片。共聚焦显微镜能够区分失焦层，以生成光学切片，这些切片可在摄影胶片上叠加以获得神经元重建图像。双平面图像是通过使用立体观察器融合用传统透射光显微镜获得的两个相邻光学切片而得到的。展示了3日龄雏鸡神经元的立体和双平面显微照片。
