Olson L, Seiger A, Hoffer B, Taylor D
Exp Brain Res. 1979 Mar 9;35(1):47-67. doi: 10.1007/BF00236784.
Sequential intraocular grafting of defined areas from fetal rat brain to adult host rats was used to explore the possibility that such double grafts would become interconnected. Norepinephrine- containing neurons of the locus coeruleus were grafted together with either parietal cerebral cortex, hippocampus, or the caudate nucleus. Dopamine-containing neurons of the substantia nigra were transplanted together with either parietal cerebral cortex or the caudate nucleus. The brainstem grafts showed good survival and development in oculo, using both histochemical and electrophysiological criteria. Locus coeruleus neurons were found to innervate cerebral cortex, hippocampus, and the caudate nucleus. Substantia nigra neurons invaded cerebral cortex abundantly, with a terminal distribution typical of cortical DA terminals in situ. The innervation of the caudate nucleus from substantia nigra transplants was variable, but areas of dense confluent terminals were observed. We conclude that sequential brain grafting in oculo permits generation of isolated yet defined catecholaminergic projections, which are suitable for electrophysiological, pharmacological, and histochemical studies.
将胎鼠脑的特定区域依次眼内移植到成年宿主大鼠体内，以探究这种双重移植是否会相互连接的可能性。将蓝斑中含去甲肾上腺素的神经元与顶叶大脑皮层、海马体或尾状核一起移植。将黑质中含多巴胺的神经元与顶叶大脑皮层或尾状核一起移植。利用组织化学和电生理标准，发现脑干移植物在眼内具有良好的存活和发育情况。发现蓝斑神经元可支配大脑皮层、海马体和尾状核。黑质神经元大量侵入大脑皮层，其终末分布具有原位皮质多巴胺能终末的典型特征。黑质移植对尾状核的支配情况各不相同，但观察到有密集融合终末的区域。我们得出结论，眼内顺序性脑移植能够产生孤立但明确的儿茶酚胺能投射，适用于电生理、药理学和组织化学研究。
