Gerhardt G A, Palmer M R, Seiger A, Adams R N, Olson L, Hoffer B J
Brain Res. 1984 Jul 23;306(1-2):319-25. doi: 10.1016/0006-8993(84)90381-0.
In vivo electrochemical detection was used to study transmitter release from a synaptically or pharmacologically stimulated noradrenergic isolated pathway formed by double in oculo brain tissue grafts. Retinal illumination, which activates cholinergic nerve fibers that grow into intraocular grafts from the autonomic ground plexus of the iris, produced increases in the concentration of electroactive species in the hippocampal portion of intraocular locus coeruleus-hippocampus double grafts. This response was potentiated after cholinesterase inhibition, and mimicked by perfusion of carbachol, a muscarinic agonist. Much smaller increases in the electroactive species were measured in reserpinized animals, and this minimal response was completely eliminated by the subsequent inhibition of catecholamine synthesis with alpha-methyl-p-tyrosine. Taken together, the data suggest that synaptically released transmitter in these isolated brain circuits can be measured using in vivo electrochemical detection techniques, and lend further support to the hypothesis that in oculo locus coeruleus grafts develop a functional catecholaminergic input to sequentially grafted hippocampus.
体内电化学检测被用于研究由双眼中脑组织移植形成的经突触或药理学刺激的去甲肾上腺素能分离通路的递质释放。视网膜光照可激活从虹膜自主神经丛长入眼内移植体的胆碱能神经纤维，从而使眼内蓝斑-海马双移植体海马部分的电活性物质浓度增加。胆碱酯酶抑制后这种反应增强，且毒蕈碱激动剂卡巴胆碱灌注可模拟该反应。在利血平化的动物中测量到电活性物质的增加要小得多，而随后用α-甲基-对-酪氨酸抑制儿茶酚胺合成可完全消除这种最小反应。综上所述，数据表明这些分离脑回路中经突触释放的递质可用体内电化学检测技术进行测量，并进一步支持了以下假说：眼中蓝斑移植体向依次移植的海马形成功能性儿茶酚胺能输入。
