Röder A, Koschel K
J Virol. 1974 Oct;14(4):846-52. doi: 10.1128/JVI.14.4.846-852.1974.
In poliovirus-infected HeLa-S3 cells, the protease inhibitors tolylsulfonyl-phenylalanyl chloromethyl ketone and iodoacetamide cause an accumulation of large precursor proteins, and they block viral RNA synthesis most probably via these products. Viral RNA polymerase activity can, however, be extracted by detergent containing buffer (Tris/Nonidet P-40, deoxycholate) from the inhibited cells. Only cytoplasmic extracts from infected cells treated with tolylsulfonyl-phenylalanyl chloromethyl ketone or iodoacetamide contain a protein which inhibits the in vitro polymerase reaction.
在脊髓灰质炎病毒感染的HeLa-S3细胞中，蛋白酶抑制剂甲苯磺酰苯丙氨酰氯甲基酮和碘乙酰胺会导致大量前体蛋白积累，并且它们很可能通过这些产物阻断病毒RNA合成。然而，病毒RNA聚合酶活性可以用含去污剂的缓冲液（Tris/Nonidet P-40、脱氧胆酸盐）从受抑制的细胞中提取出来。只有用甲苯磺酰苯丙氨酰氯甲基酮或碘乙酰胺处理过的感染细胞的细胞质提取物中含有一种能抑制体外聚合酶反应的蛋白质。
