脊髓灰质炎病毒核糖核酸的体外合成：复制中间体的作用。
In vitro synthesis of poliovirus ribonucleic acid: role of the replicative intermediate.
作者信息
Girard M
出版信息
J Virol. 1969 Apr;3(4):376-84. doi: 10.1128/JVI.3.4.376-384.1969.
Abstract
Poliovirus ribonucleic acid (RNA) polymerase crude extracts could be stored frozen in liquid nitrogen without loss of activity or specificity. The major in vitro product of these extracts was viral single-stranded RNA. However, after short periods of incubation with radioactive nucleoside triphosphates, most of the incorporated label was found in replicative intermediate. When excess unlabeled nucleoside triphosphate was added, the label was displaced from the replicative intermediate and accumulated as viral RNA. It is concluded from this experiment that the replicative intermediate is the precursor to viral RNA. In addition, some of the label was chased into double-stranded RNA. The implications of this finding are discussed.
摘要
脊髓灰质炎病毒核糖核酸（RNA）聚合酶粗提物可冷冻保存在液氮中，而不会丧失活性或特异性。这些提取物的主要体外产物是病毒单链RNA。然而，在用放射性核苷三磷酸短暂孵育后，发现大部分掺入的标记物存在于复制中间体中。当加入过量的未标记核苷三磷酸时，标记物从复制中间体中被取代，并作为病毒RNA积累。从该实验得出的结论是，复制中间体是病毒RNA的前体。此外，一些标记物被追踪到双链RNA中。讨论了这一发现的意义。
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