Trowbridge R S
Appl Microbiol. 1974 Sep;28(3):366-73. doi: 10.1128/am.28.3.366-373.1974.
A simple and direct plaque assay for maedi virus, two strains of progressive pneumonia virus, and two strains of visna virus has been developed and evaluated. The technique allows the plaques formed by these viruses to be localized without disturbing the host-cell substrate of sheep choroid plexus cells or the gelled maintenance medium over the host-cell monolayer. Diethylaminoethyl-dextran supplementation of the medium used to overlay strain K796 visna virus-infected cultures decreases the time required for maximum plaque development from 12 to 10 days, enhances the contrast of the plaques, increases the titer of plaque-forming units, and permits a plaque size heterogeneity to be realized. Both large and small plaques occur in cultures infected with the visna viruses, one strain of progressive pneumonia virus, or maedi virus. In contrast, the plaques observed in cultures infected with the second strain of progressive pneumonia virus are relatively homogeneous in size.
已开发并评估了一种用于梅迪病毒、两株进行性肺炎病毒和两株维斯纳病毒的简单直接的蚀斑测定法。该技术能使这些病毒形成的蚀斑定位，而不会干扰绵羊脉络丛细胞的宿主细胞底物或宿主细胞单层上的凝胶化维持培养基。用于覆盖感染K796株维斯纳病毒培养物的培养基中添加二乙氨基乙基葡聚糖，可将最大蚀斑形成所需时间从12天缩短至10天，增强蚀斑对比度，提高蚀斑形成单位的滴度，并使蚀斑大小异质性得以实现。在感染维斯纳病毒、一株进行性肺炎病毒或梅迪病毒的培养物中会出现大、小两种蚀斑。相比之下，在感染第二株进行性肺炎病毒的培养物中观察到的蚀斑大小相对均匀。
