甘氨酸突触受体：士的宁结合与离子电导机制相关的证据。
The glycine synaptic receptor: evidence that strychnine binding is associated with the ionic conductance mechanism.
作者信息
Young A B, Snyder S H
出版信息
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4002-5. doi: 10.1073/pnas.71.10.4002.
Abstract
The ability of a series of anions to inhibit [(3)H]strychnine binding to spinal cord synaptic membranes correlates closely with their neurophysiologic capacity to reverse inhibitory postsynaptic potentials in the mammalian spinal cord. Seven neurophysiologically active anions are also effective inhibitors of [(3)H]strychnine binding with mean effective doses ranging from 160 to 620 mM. Seven other anions that are ineffective neurophysiologically also fail to alter strychnine binding. Chloride inhibits strychnine binding in a noncompetitive fashion. Hill plots of the displacement of [(3)H]strychnine by chloride give coefficients of 2.3-2.7. The inhibition of strychnine binding by these anions suggests that strychnine binding is closely associated with the ionic conductance mechanism for chloride in the glycine receptor.
摘要
一系列阴离子抑制[³H]士的宁与脊髓突触膜结合的能力，与其在哺乳动物脊髓中逆转抑制性突触后电位的神经生理能力密切相关。七种具有神经生理活性的阴离子也是[³H]士的宁结合的有效抑制剂，平均有效剂量范围为160至620 mM。其他七种在神经生理学上无效的阴离子也不能改变士的宁的结合。氯离子以非竞争性方式抑制士的宁结合。氯离子对[³H]士的宁置换的希尔图给出的系数为2.3 - 2.7。这些阴离子对士的宁结合的抑制表明，士的宁结合与甘氨酸受体中氯离子的离子传导机制密切相关。
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