士的宁结合与中枢神经系统的甘氨酸受体相关。
Strychnine binding associated with glycine receptors of the central nervous system.
作者信息
Young A B, Snyder S H
出版信息
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2832-6. doi: 10.1073/pnas.70.10.2832.
Abstract
[(3)H]Strychnine binds to synaptic-membrane fractions of the spinal cord in a selective fashion, indicating an interaction with postsynaptic glycine receptors. Displacement of strychnine by glycine and other amino acids parallels their glycine-like neurophysiologic activity. The regional localization of strychnine binding in the central nervous system correlates closely with endogenous glycine concentrations. In subcellular fractionation experiments, strychnine binding is most enhanced in synaptic-membrane fractions. Strychnine binding is saturable, with affinity constants for glycine and strychnine of 10 and 0.03 muM, respectively.
摘要
[（3）H]士的宁以一种选择性方式与脊髓的突触膜部分结合，表明其与突触后甘氨酸受体相互作用。甘氨酸和其他氨基酸对士的宁的取代作用与其类似甘氨酸的神经生理活性平行。士的宁在中枢神经系统中的结合区域定位与内源性甘氨酸浓度密切相关。在亚细胞分级分离实验中，突触膜部分的士的宁结合增强最为明显。士的宁结合具有饱和性，甘氨酸和士的宁的亲和常数分别为10和0.03μM。
相似文献
1
Strychnine binding associated with glycine receptors of the central nervous system.士的宁结合与中枢神经系统的甘氨酸受体相关。
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2832-6. doi: 10.1073/pnas.70.10.2832.
2
Glycine high affinity uptake and strychnine binding associated with glycine receptors in the frog central nervous system.青蛙中枢神经系统中与甘氨酸受体相关的甘氨酸高亲和力摄取及士的宁结合
Brain Res. 1978 Mar 31;143(3):487-98. doi: 10.1016/0006-8993(78)90359-1.
3
The glycine synaptic receptor: evidence that strychnine binding is associated with the ionic conductance mechanism.甘氨酸突触受体：士的宁结合与离子电导机制相关的证据。
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4002-5. doi: 10.1073/pnas.71.10.4002.
4
Interaction of benzodiazepines with central nervous glycine receptors: possible mechanism of action.苯二氮䓬类药物与中枢神经系统甘氨酸受体的相互作用：可能的作用机制。
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2246-50. doi: 10.1073/pnas.71.6.2246.
5
Synaptic biochemistry of amino acids.氨基酸的突触生物化学
Fed Proc. 1973 Oct;32(10):2039-47.
6
Fluorescent labelling of strychnine : a novel approach for recognition of strychnine binding sites on neuronal membrane.士的宁的荧光标记：一种识别神经元膜上士的宁结合位点的新方法。
Biochem Biophys Res Commun. 1981 Jul 16;101(1):273-80. doi: 10.1016/s0006-291x(81)80041-1.
7
Binding of [3H]glycine, [3H]beta-alanine and [3H]strychnine in cultured rat spinal cord and brain stem.[3H]甘氨酸、[3H]β-丙氨酸和[3H]士的宁在培养的大鼠脊髓和脑干中的结合。
Brain Res. 1981 May 25;213(1):242-5. doi: 10.1016/0006-8993(81)91270-1.
8
Strychinine binding associated with synaptic glycine receptors in rat spinal cord membranes: ionic influences.大鼠脊髓膜中与突触甘氨酸受体相关的士的宁结合：离子影响
Brain Res. 1978 May 19;147(1):107-16. doi: 10.1016/0006-8993(78)90775-8.
9
Stereospecific interaction of bicuculline with specific [3H]strychnine binding to rat spinal cord synaptosomal membranes.荷包牡丹碱与特异性[³H]士的宁结合大鼠脊髓突触体膜的立体特异性相互作用。
Neurosci Lett. 1980 Jan;16(1):91-5. doi: 10.1016/0304-3940(80)90107-x.
10
Characterization of [3H]thiocolchicoside binding sites in rat spinal cord and cerebral cortex.大鼠脊髓和大脑皮层中[3H]硫代秋水仙碱结合位点的表征
Eur J Pharmacol. 1999 Jul 2;376(1-2):149-57. doi: 10.1016/s0014-2999(99)00371-4.
引用本文的文献
1
HPLC-ESI-HRMS/MS-Based Metabolite Profiling and Bioactivity Assessment of .基于高效液相色谱-电喷雾电离-高分辨质谱/质谱的代谢物谱分析及生物活性评估
Plants (Basel). 2025 Aug 2;14(15):2395. doi: 10.3390/plants14152395.
2
Investigation of central pattern generators in the spinal cord of chicken embryos.鸡胚脊髓中中枢模式发生器的研究。
J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2024 Sep;210(5):801-814. doi: 10.1007/s00359-024-01694-6. Epub 2024 Mar 23.
3
Aminomethanesulfonic acid illuminates the boundary between full and partial agonists of the pentameric glycine receptor.甲氨磺酸阐明了五聚体甘氨酸受体完全激动剂和部分激动剂之间的界限。
Elife. 2022 Aug 17;11:e79148. doi: 10.7554/eLife.79148.
4
Molecular Mingling: Multimodal Predictions of Ligand Promiscuity in Pentameric Ligand-Gated Ion Channels.分子交融：五聚体配体门控离子通道中配体多特异性的多模态预测
Front Mol Biosci. 2022 May 9;9:860246. doi: 10.3389/fmolb.2022.860246. eCollection 2022.
5
An Electrophysiological and Pharmacological Study of the Properties of Human iPSC-Derived Neurons for Drug Discovery.人类诱导多能干细胞衍生神经元的电生理和药理学特性的研究及其在药物发现中的应用。
Cells. 2021 Jul 31;10(8):1953. doi: 10.3390/cells10081953.
6
Subunit-Specific Photocontrol of Glycine Receptors by Azobenzene-Nitrazepam Photoswitcher.通过偶氮苯-硝基安定光开关对甘氨酸受体的亚基特异性光控。
eNeuro. 2021 Jan 21;8(1). doi: 10.1523/ENEURO.0294-20.2020. Print 2021 Jan-Feb.
7
Comparing Cryo-EM Reconstructions and Validating Atomic Model Fit Using Difference Maps.比较冷冻电镜重建并使用差异图验证原子模型拟合
J Chem Inf Model. 2020 May 26;60(5):2552-2560. doi: 10.1021/acs.jcim.9b01103. Epub 2020 Feb 11.
8
Glycine Metabolism and Its Alterations in Obesity and Metabolic Diseases.甘氨酸代谢及其在肥胖和代谢性疾病中的改变。
Nutrients. 2019 Jun 16;11(6):1356. doi: 10.3390/nu11061356.
9
Impact of Rodenticides on the Coagulation Properties of Milk.杀鼠剂对牛奶凝血特性的影响。
Foods. 2018 Apr 7;7(4):57. doi: 10.3390/foods7040057.
10
Voltage-Dependent Inhibition of Glycine Receptor Channels by Niflumic Acid.氟尼辛对甘氨酸受体通道的电压依赖性抑制作用
Front Mol Neurosci. 2017 May 16;10:125. doi: 10.3389/fnmol.2017.00125. eCollection 2017.
本文引用的文献
1
Observations on strychnine reversal.关于士的宁逆转的观察
J Physiol. 1911 Nov 20;43(3-4):232-41. doi: 10.1113/jphysiol.1911.sp001469.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
An investigation of primary or direct inhibition.对初级或直接抑制的研究。
J Physiol. 1953 Dec 29;122(3):474-88. doi: 10.1113/jphysiol.1953.sp005015.
4
Distribution of some synaptic transmitter suspects in cat spinal cord: glutamic acid, aspartic acid, gamma-aminobutyric acid, glycine and glutamine.猫脊髓中一些疑似突触递质的分布：谷氨酸、天冬氨酸、γ-氨基丁酸、甘氨酸和谷氨酰胺。
J Neurochem. 1967 Apr;14(4):465-72. doi: 10.1111/j.1471-4159.1967.tb09545.x.
5
Regional studies of catecholamines in the rat brain. I. The disposition of [3H]norepinephrine, [3H]dopamine and [3H]dopa in various regions of the brain.大鼠脑中儿茶酚胺的区域研究。I. [3H]去甲肾上腺素、[3H]多巴胺和[3H]多巴在脑不同区域的分布。
J Neurochem. 1966 Aug;13(8):655-69. doi: 10.1111/j.1471-4159.1966.tb09873.x.
6
The distribution of glycine in cat spinal cord and roots.甘氨酸在猫脊髓和神经根中的分布。
Life Sci. 1965 Nov;4(21):2075-83. doi: 10.1016/0024-3205(65)90325-5.
7
A comparison of the concentration of glycine, a transmitter suspect, in different areas of the brain and spinal cord in seven different vertebrates.对七种不同脊椎动物大脑和脊髓不同区域中疑似神经递质甘氨酸浓度的比较。
Comp Biochem Physiol. 1969 Mar;28(3):1345-55. doi: 10.1016/0010-406x(69)90571-4.
8
A pharmacological study of the depression of spinal neurones by glycine and related amino acids.甘氨酸及相关氨基酸对脊髓神经元抑制作用的药理学研究。
Exp Brain Res. 1968;6(1):1-18. doi: 10.1007/BF00235443.
9
The nitrogen content of the water soluble proteins of three species of the free-living amoebae.三种自由生活变形虫的水溶性蛋白质中的氮含量。
Life Sci. 1968 Jun 15;7(12):583-5. doi: 10.1016/0024-3205(68)90078-7.
10
Insulin--receptor interactions in adipose tissue cells: direct measurement and properties.脂肪组织细胞中的胰岛素 - 受体相互作用：直接测量与特性
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1264-8. doi: 10.1073/pnas.68.6.1264.
Zotero 插件