Etchison J R, Holland J J
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4011-4. doi: 10.1073/pnas.71.10.4011.
The carbohydrate composition of the membrane glycoprotein of vesicular stomatitis virus has been determined for virus grown in four different mammalian cell lines. The glycoprotein contains mannose, galactose, N-acetylglucosamine, and neuraminic acid as the major carbohydrate components, whereas N-acetyl-galactosamine and fucose are present in lesser amounts. The glycoprotein contains approximately 9-10% carbohydrate regardless of the host cell in which it is synthesized. Small quantitative differences are evident in the composition of the component sugars of the glycoprotein when the virus is grown in different host cells, and the glycoprotein of virus grown in a mouse fibroblast line (L cells) lacks fucose. The major oligosaccharide moieties of the virus glycoprotein from all cells are approximately the same size (3000-3400 daltons). The data presented here, in conjunction with previous data, indicate that the viral glycoprotein contains two major oligosaccharide constituents regardless of the host cell in which it is synthesized.
已测定了水泡性口炎病毒膜糖蛋白的碳水化合物组成，该病毒是在四种不同的哺乳动物细胞系中培养出来的。这种糖蛋白含有甘露糖、半乳糖、N-乙酰葡糖胺和神经氨酸作为主要的碳水化合物成分，而N-乙酰半乳糖胺和岩藻糖的含量较少。无论在何种宿主细胞中合成，这种糖蛋白都含有约9-10%的碳水化合物。当病毒在不同的宿主细胞中培养时，糖蛋白组成糖成分的含量存在明显的微小差异，在小鼠成纤维细胞系（L细胞）中培养的病毒糖蛋白不含岩藻糖。来自所有细胞的病毒糖蛋白的主要寡糖部分大小大致相同（3000-3400道尔顿）。此处呈现的数据与之前的数据表明，无论在何种宿主细胞中合成，病毒糖蛋白都含有两种主要的寡糖成分。
