大鼠肾脏中胞饮过程的分析。I. 从大鼠肾皮质分离胞饮小泡。
Analysis of the pinocytic process in rat kidney. I. Isolation of pinocytic vesicles from rat kidney cortex.
作者信息
Bode F, Pockrandt-Hemstedt H, Baumann K, Kinne R
出版信息
J Cell Biol. 1974 Dec;63(3):998-1008. doi: 10.1083/jcb.63.3.998.
Abstract
Pinocytosis was induced in rat kidney by exposure to horseradish peroxidase (HRP). Pinocytic vesicle preparations were enriched after homogenization of kidney cortex by differential centrifugation and free-flow electrophoresis with HRP as an exogenous marker. Vesicles were identified by enzymatic analysis and by electron microscopy, including specific staining procedures. Typical brush-border enzymes such as alkaline phosphatase, aminopeptidase, 5'-nucleotidase, lysosomal acid phosphatase, and mitochondrial succinic dehydrogenase were reduced in the vesicular fraction, compared to the kidney cortex homogenate. Glucose-6-phosphatase and Na(+)-K(+)-ATPase were only slightly increased in the fraction. These results indicate that preparations of pinocytic vesicles from rat kidney cortex can be enriched. They have biochemical characteristics that differ from those of the cell organelles and membranes previously purified from renal tissue.
摘要
通过暴露于辣根过氧化物酶（HRP）诱导大鼠肾脏发生胞饮作用。以HRP作为外源性标记物，经差速离心和自由流动电泳对肾皮质进行匀浆后，富集胞饮小泡制剂。通过酶分析和电子显微镜（包括特定染色程序）鉴定小泡。与肾皮质匀浆相比，典型的刷状缘酶如碱性磷酸酶、氨肽酶、5'-核苷酸酶、溶酶体酸性磷酸酶和线粒体琥珀酸脱氢酶在小泡组分中减少。葡萄糖-6-磷酸酶和钠钾ATP酶在该组分中仅略有增加。这些结果表明，可以富集大鼠肾皮质的胞饮小泡制剂。它们具有与先前从肾组织中纯化的细胞器和膜不同的生化特性。
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