产气克雷伯菌野生型和突变型核糖醇脱氢酶的比较。
A comparison of wild-type and mutant ribitol dehydrogenases from Klebsiella aerogenes.
作者信息
Burleigh B D, Rigby P W, Hartley B S
出版信息
Biochem J. 1974 Nov;143(2):341-52. doi: 10.1042/bj1430341.
Abstract
A ribitol dehydrogenase (ribitol-NAD(+) oxidoreductase, EC. 1.1.1.56) having increased specificity and catalytic efficiency toward xylitol was isolated from mutant strains of Klebsiella aerogenes, which were selected for increased growth rate on xylitol over the ribitol dehydrogenase constitutive wild-type organism. 2. The mutant enzyme was purified to homogeneity and its general characteristics were compared with those of the previously purified wild-type enzyme. 3. Initial-velocity steady-state kinetic parameters were determined for both wild-type and mutant enzymes and the results compared. 4. The results are interpreted in terms of a model in which the mutant enzyme results from a small change of amino acid sequence, which affects both the stability and conformational equilibria of the molecule.
摘要
- 从产气克雷伯菌的突变菌株中分离出一种对木糖醇具有更高特异性和催化效率的核糖醇脱氢酶（核糖醇-NAD(+)氧化还原酶，EC. 1.1.1.56），这些突变菌株是通过在木糖醇上比核糖醇脱氢酶组成型野生型生物体具有更高的生长速率而筛选出来的。2. 将突变酶纯化至同质，并将其一般特性与先前纯化的野生型酶的特性进行比较。3. 测定了野生型和突变型酶的初速度稳态动力学参数，并比较了结果。4. 根据一个模型对结果进行了解释，在该模型中，突变酶是由氨基酸序列的微小变化产生的，这影响了分子的稳定性和构象平衡。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
3
EVOLUTION OF A CATABOLIC PATHWAY IN BACTERIA.细菌中分解代谢途径的进化
Science. 1964 Dec 4;146(3649):1313-5. doi: 10.1126/science.146.3649.1313.
4
The purification of nicotinamide adenine dinucleotide and kinetic effects of nucleotide impurities.烟酰胺腺嘌呤二核苷酸的纯化及核苷酸杂质的动力学效应
J Biol Chem. 1963 Apr;238:1538-43.
5
The kinetics of enzyme-catalyzed reactions with two or more substrates or products. I. Nomenclature and rate equations.具有两种或更多种底物或产物的酶催化反应动力学。I. 命名法和速率方程。
Biochim Biophys Acta. 1963 Jan 8;67:104-37. doi: 10.1016/0006-3002(63)91800-6.
6
Graphical determination of the dissociation constants for two-substrate enzyme systems.双底物酶系统解离常数的图形测定法。
Biochim Biophys Acta. 1957 Sep;25(3):575-8. doi: 10.1016/0006-3002(57)90529-2.
7
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
8
Mutants of Aerobacter aerogenes capable of utilizing xylitol as a novel carbon.产气气杆菌中能够利用木糖醇作为新型碳源的突变体。
J Bacteriol. 1968 Aug;96(2):447-56. doi: 10.1128/jb.96.2.447-456.1968.
9
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
10
A basis for utlization of unnatural pentoses and pentitols by Aerobacter aerogenes.产气气杆菌利用非天然戊糖和戊糖醇的基础。
Proc Natl Acad Sci U S A. 1965 Aug;54(2):572-9. doi: 10.1073/pnas.54.2.572.
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