Doten R C, Mortlock R P
J Bacteriol. 1985 May;162(2):845-8. doi: 10.1128/jb.162.2.845-848.1985.
Morganella morganii ATCC 25829, Providencia stuartii ATCC 25827, Serratia marcescens ATCC 13880, and Erwinia sp. strain 4D2P were found to induce a xylitol dehydrogenase when grown on a xylitol-containing medium. The xylitol dehydrogenases were partially purified from the four strains, and those from M. morganii ATCC 25829, P. stuartii ATCC 25827, and S. marcescens ATCC 13880 were all found to oxidize xylitol to D-xylulose. These three enzymes had KmS for xylitol of 7.1 to 16.4 mM and molecular weights ranging from 130,000 to 155,000. In contrast, the xylitol dehydrogenase from Erwinia sp. strain 4D2P oxidized xylitol at the C-4 position to produce L-xylulose, had a Km for xylitol of 72 mM, and had a molecular weight of 102,000.
摩根氏摩根菌ATCC 25829、斯氏普罗威登斯菌ATCC 25827、粘质沙雷氏菌ATCC 13880和欧文氏菌属菌株4D2P在含木糖醇的培养基上生长时被发现可诱导产生木糖醇脱氢酶。从这四种菌株中对木糖醇脱氢酶进行了部分纯化，并且发现来自摩根氏摩根菌ATCC 25829、斯氏普罗威登斯菌ATCC 25827和粘质沙雷氏菌ATCC 13880的木糖醇脱氢酶均能将木糖醇氧化为D-木酮糖。这三种酶对木糖醇的米氏常数为7.1至16.4 mM，分子量在130,000至155,000之间。相比之下，欧文氏菌属菌株4D2P的木糖醇脱氢酶在C-4位将木糖醇氧化生成L-木酮糖，其对木糖醇的米氏常数为72 mM，分子量为102,000。
