腺苷酰亚胺二磷酸对线粒体可溶性三磷酸腺苷酶的抑制作用。
Inhibition of the soluble adenosine triphosphatase from mitochondria by adenylyl imidodiphosphate.
作者信息
Philo R D, Selwyn M J
出版信息
Biochem J. 1974 Dec;143(3):745-9. doi: 10.1042/bj1430745.
Abstract
- Adenylyl imidodiphosphate is an inhibitor with high affinity for the soluble ATPase (adenosine triphosphatase) from mitochondria. 2. The reaction of the inhibitor with the ATPase is slow and estimates for the association and dissociation reaction rate constants are given. 3. The number of binding sites for the inhibitor appears to be doubled in the presence of 2,4-dinitrophenol. 4. Adenylyl imidodiphosphate is less effective as an inhibitor of the ATPase activity of this enzyme than of the inosine triphosphatase activity. It is also less effective on the ATPase of frozen-thawed or intact mitochondria and did not inhibit ADP-stimulated respiration by intact mitochondria.
摘要
- 腺苷亚氨二磷酸是一种对线粒体可溶性ATP酶（腺苷三磷酸酶）具有高亲和力的抑制剂。2. 抑制剂与ATP酶的反应缓慢，并给出了结合和解离反应速率常数的估计值。3. 在2,4-二硝基苯酚存在的情况下，抑制剂的结合位点数量似乎增加了一倍。4. 腺苷亚氨二磷酸作为该酶ATP酶活性的抑制剂，其效果不如对肌苷三磷酸酶活性的抑制效果。它对冻融或完整线粒体的ATP酶也不太有效，并且不抑制完整线粒体的ADP刺激的呼吸作用。
相似文献
1
Inhibition of the soluble adenosine triphosphatase from mitochondria by adenylyl imidodiphosphate.腺苷酰亚胺二磷酸对线粒体可溶性三磷酸腺苷酶的抑制作用。
Biochem J. 1974 Dec;143(3):745-9. doi: 10.1042/bj1430745.
2
Exploring sites on mitochondrial ATPase for catalysis, regulation, and inhibition.探索线粒体ATP酶上的催化、调节和抑制位点。
J Supramol Struct. 1975;3(3):214-21. doi: 10.1002/jss.400030303.
3
Characteristics of adenylyl imidodiphosphate- and ADP-binding sites insoluble and particulate mitochondrial ATPase. Studies with methanol.腺苷酰亚胺二磷酸和ADP结合位点不溶性及颗粒状线粒体ATP酶的特性。甲醇研究。
Biochim Biophys Acta. 1982 Mar 16;679(3):466-73. doi: 10.1016/0005-2728(82)90168-2.
4
Comparison of effects of inhibitors on adenosine triphosphatase and adenosine diphosphatase activities in rat-liver mitochondria.抑制剂对大鼠肝脏线粒体中三磷酸腺苷酶和二磷酸腺苷酶活性影响的比较。
Eur J Biochem. 1984 Mar 15;139(3):529-34. doi: 10.1111/j.1432-1033.1984.tb08037.x.
5
A kinetic study of the interaction between mitochondrial F1 adenosine triphosphatase and adenylyl imidodiphosphate and guanylyl imidodiphosphate.线粒体F1三磷酸腺苷酶与腺苷酰亚胺二磷酸和鸟苷酰亚胺二磷酸相互作用的动力学研究。
Biochem J. 1983 Mar 15;210(3):727-35. doi: 10.1042/bj2100727.
6
Action of the adenosine triphosphate analog, adenylyl imidodiphosphate in mitochondria.
Arch Biochem Biophys. 1975 Jan;166(1):139-44. doi: 10.1016/0003-9861(75)90373-2.
7
Citreoviridin, a specific inhibitor of the mitochondiral adenosine triphosphatase.黄绿青霉素，一种线粒体腺苷三磷酸酶的特异性抑制剂。
Biochem J. 1978 Mar 15;170(3):503-10. doi: 10.1042/bj1700503.
8
Azide inhibition of mitochondrial ATPase.
Biochem Biophys Res Commun. 1968 Jan 11;30(1):83-8. doi: 10.1016/0006-291x(68)90716-x.
9
Deficiency of uncoupler-stimulated adenosine triphosphatase activity in tightly coupled hepatoma mitochondria.紧密偶联的肝癌线粒体中解偶联剂刺激的三磷酸腺苷酶活性缺乏。
Proc Natl Acad Sci U S A. 1971 May;68(5):1079-82. doi: 10.1073/pnas.68.5.1079.
10
Kinetic studies of beef heart mitochondrial adenosine triphosphatase: interaction of the inhibitor protein and adenosine triphosphate analogues.牛心线粒体三磷酸腺苷酶的动力学研究：抑制蛋白与三磷酸腺苷类似物的相互作用
Biochemistry. 1981 Mar 17;20(6):1592-8. doi: 10.1021/bi00509a028.
引用本文的文献
1
Kinetic and thermodynamic properties of beef heart mitochondrial ATPase: effect of co-solvent systems.牛心线粒体ATP酶的动力学和热力学性质：共溶剂体系的影响。
J Bioenerg Biomembr. 1980 Dec;12(5-6):369-78. doi: 10.1007/BF00748765.
2
A kinetic study of the interaction between mitochondrial F1 adenosine triphosphatase and adenylyl imidodiphosphate and guanylyl imidodiphosphate.线粒体F1三磷酸腺苷酶与腺苷酰亚胺二磷酸和鸟苷酰亚胺二磷酸相互作用的动力学研究。
Biochem J. 1983 Mar 15;210(3):727-35. doi: 10.1042/bj2100727.
3
The proton and metal complexes of adenyl-5'-yl imidodiphosphate.腺苷 -5'- 基亚氨二磷酸的质子和金属配合物。
Biochem J. 1976 Oct 1;159(1):169-71. doi: 10.1042/bj1590169.
4
Mixed anhydrides of nucleotides and mesitylenecarboxylic acid as new specific inhibitors of mitochondrial adenosien triphosphatase.核苷酸与均三甲苯羧酸的混合酸酐作为线粒体腺苷三磷酸酶的新型特异性抑制剂。
Biochem J. 1979 Feb 15;178(2):339-43. doi: 10.1042/bj1780339.
本文引用的文献
1
[Studies on cholinesterase. 7. Determination of the molar concentration of pseudo-cholinesterase in serum].[胆碱酯酶研究。7. 血清中假性胆碱酯酶摩尔浓度的测定]
Biochem J. 1952 Jun;51(3):303-11. doi: 10.1042/bj0510303.
2
Chloride-hydroxide exchange across mitochondrial, erythrocyte and artificial lipid membranes mediated by trialkyl- and triphenyltin compounds.由三烷基锡和三苯基锡化合物介导的跨线粒体、红细胞及人工脂质膜的氯-氢交换。
Eur J Biochem. 1970 May 1;14(1):120-6. doi: 10.1111/j.1432-1033.1970.tb00268.x.
3
Adenylyl imidodiphosphate, an adenosine triphosphate analog containing a P--N--P linkage.腺苷亚氨基二磷酸，一种含有P-N-P键的三磷酸腺苷类似物。
Biochemistry. 1971 Jun 22;10(13):2484-9. doi: 10.1021/bi00789a009.
4
Use of progress curves to investigate product inhibition in enzyme-catalysed reactions. Application to the soluble mitochondrial adenosine triphosphatase.利用进程曲线研究酶催化反应中的产物抑制作用。应用于可溶性线粒体三磷酸腺苷酶。
Biochem J. 1973 Nov;135(3):525-30. doi: 10.1042/bj1350525.
5
Tight binding of adenine nucleotides to beef-heart mitochondrial ATPase.
Biochim Biophys Acta. 1973 Aug 31;314(2):149-53. doi: 10.1016/0005-2728(73)90130-8.
Zotero 插件