腺苷酰亚胺二磷酸对线粒体可溶性三磷酸腺苷酶的抑制作用。
Inhibition of the soluble adenosine triphosphatase from mitochondria by adenylyl imidodiphosphate.
作者信息
Philo R D, Selwyn M J
出版信息
Biochem J. 1974 Dec;143(3):745-9. doi: 10.1042/bj1430745.
Abstract
- Adenylyl imidodiphosphate is an inhibitor with high affinity for the soluble ATPase (adenosine triphosphatase) from mitochondria. 2. The reaction of the inhibitor with the ATPase is slow and estimates for the association and dissociation reaction rate constants are given. 3. The number of binding sites for the inhibitor appears to be doubled in the presence of 2,4-dinitrophenol. 4. Adenylyl imidodiphosphate is less effective as an inhibitor of the ATPase activity of this enzyme than of the inosine triphosphatase activity. It is also less effective on the ATPase of frozen-thawed or intact mitochondria and did not inhibit ADP-stimulated respiration by intact mitochondria.
摘要
- 腺苷亚氨二磷酸是一种对线粒体可溶性ATP酶（腺苷三磷酸酶）具有高亲和力的抑制剂。2. 抑制剂与ATP酶的反应缓慢，并给出了结合和解离反应速率常数的估计值。3. 在2,4-二硝基苯酚存在的情况下，抑制剂的结合位点数量似乎增加了一倍。4. 腺苷亚氨二磷酸作为该酶ATP酶活性的抑制剂，其效果不如对肌苷三磷酸酶活性的抑制效果。它对冻融或完整线粒体的ATP酶也不太有效，并且不抑制完整线粒体的ADP刺激的呼吸作用。
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