大鼠肝脏颗粒组分的生物化学与形态学联合研究。富含溶酶体或含有尿酸氧化酶、D-氨基酸氧化酶和过氧化氢酶颗粒的制剂分析。
Combined biochemical and morphological study of particulate fractions from rat liver. Analysis of preparations enriched in lysosomes or in particles containing urate oxidase, D-amino acid oxidase, and catalase.
作者信息
Baudhuin P, Beaufay H, De Duve C
出版信息
J Cell Biol. 1965 Jul;26(1):219-43. doi: 10.1083/jcb.26.1.219.
Abstract
Six particulate preparations isolated from rat liver under different experimental conditions were analyzed biochemically and examined in the electron microscope. The results confirm the lysosomal nature of the pericanalicular dense bodies and demonstrate that the microbodies are the bearers of urate oxidase, catalase, and D-amino acid oxidase. Catalase, representing a major component of the particles, and D-amino acid oxidase appear to be associated with the structureless "sap" of the particles, urate oxidase with their crystalloid core or with their outer membrane.
摘要
对在不同实验条件下从大鼠肝脏中分离出的六种颗粒制剂进行了生化分析，并在电子显微镜下进行了检查。结果证实了胆小管周围致密小体的溶酶体性质，并表明微体是尿酸氧化酶、过氧化氢酶和D-氨基酸氧化酶的载体。过氧化氢酶作为颗粒的主要成分，与D-氨基酸氧化酶似乎与颗粒无结构的“液汁”相关，尿酸氧化酶则与它们的晶体核心或外膜相关。
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Combined biochemical and morphological study of particulate fractions from rat liver. Analysis of preparations enriched in lysosomes or in particles containing urate oxidase, D-amino acid oxidase, and catalase.大鼠肝脏颗粒组分的生物化学与形态学联合研究。富含溶酶体或含有尿酸氧化酶、D-氨基酸氧化酶和过氧化氢酶颗粒的制剂分析。
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本文引用的文献
1
2
The liver cell. Some new approaches to its study.肝细胞。对其研究的一些新方法。
Am J Med. 1960 Jul;29:102-31. doi: 10.1016/0002-9343(60)90011-5.
3
[The bile canaliculi; study with an electronic microscope].[胆小管；电子显微镜研究]
C R Seances Soc Biol Fil. 1954 Dec;148(23-24):2008-11.
4
URICASE: LOCALIZATION IN HEPATIC MICROBODIES.尿酸氧化酶：在肝脏微体中的定位
Science. 1964 Dec 4;146(3649):1316-8. doi: 10.1126/science.146.3649.1316.
5
ELECTRON MICROSCOPIC OBSERVATIONS ON THE LOCALIZATION OF ACID PHOSPHATASE IN MOUSE HEPATIC PARENCHYMAL CELLS.小鼠肝实质细胞酸性磷酸酶定位的电子显微镜观察
Exp Cell Res. 1964 Feb;33:598-601. doi: 10.1016/0014-4827(64)90030-8.
6
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.在电子显微镜检查中，将高pH值的柠檬酸铅用作电子不透明染色剂。
J Cell Biol. 1963 Apr;17(1):208-12. doi: 10.1083/jcb.17.1.208.
7
The kinetics of catalase synthesis and destruction in vivo.过氧化氢酶在体内合成与分解的动力学。
J Biol Chem. 1962 Nov;237:3468-75.
8
[An electron microscopic examination of purified fractions of cytoplasmic organites of rat liver].[大鼠肝脏细胞质细胞器纯化组分的电子显微镜检查]
Arch Int Physiol Biochim. 1963 Jan;71:119-20.
9
The localization of acid phosphatase in rat liver cells as revealed by combined cytochemical staining and electron microscopy.通过细胞化学染色和电子显微镜联合技术揭示大鼠肝细胞中酸性磷酸酶的定位。
J Biophys Biochem Cytol. 1961 Oct;11(1):47-66. doi: 10.1083/jcb.11.1.47.
10
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