Hurlimann J, Thorbecke G J, Hochwald G M
J Exp Med. 1966 Feb 1;123(2):365-78. doi: 10.1084/jem.123.2.365.
The site of formation of C-reactive protein (CxRP, CRP) has been studied with tissues from rabbits, monkeys, and human beings. Rabbits and monkeys were stimulated to produce the acute phase protein by injection of turpentine, croton oil, endotoxin, paratyphoid-typhoid vaccine, or pneumococci. C(14)-amino acid incorporation in vitro was demonstrated by means of autoradiography of immunoelectrophoretic patterns made with culture fluids. It was found that among many different tissues tested liver was the only tissue which incorporated C(14)-lysine and isoleucine into CxRP or CRP. Only livers taken 16 to 24 hr after various stimuli were active; livers from normal animals or from animals killed 3 to 9 hr after stimulation did not produce detectable amounts of CxRP. Inflamed muscle from the injection site did not show C(14)-amino acid incorporation into CxRP. Several human tissues were also cultured, and a few liver cultures found to contain labeled CRP. The formation of CxRP or CRP by the liver was always accompanied by enhanced C(14)-amino acid incorporation into other serum proteins, but the reverse was not always found.
已利用来自兔子、猴子和人类的组织对C反应蛋白（CxRP，CRP）的形成部位进行了研究。通过注射松节油、巴豆油、内毒素、副伤寒 - 伤寒疫苗或肺炎球菌刺激兔子和猴子产生急性期蛋白。利用培养液制作免疫电泳图谱的放射自显影法证明了体外C(14) - 氨基酸的掺入。研究发现，在所测试的许多不同组织中，肝脏是唯一能将C(14) - 赖氨酸和异亮氨酸掺入CxRP或CRP的组织。只有在各种刺激后16至24小时取出的肝脏才有活性；正常动物的肝脏或刺激后3至9小时处死的动物的肝脏均未产生可检测量的CxRP。注射部位的发炎肌肉未显示C(14) - 氨基酸掺入CxRP。还培养了几种人体组织，发现少数肝脏培养物含有标记的CRP。肝脏形成CxRP或CRP时，总是伴随着C(14) - 氨基酸更多地掺入其他血清蛋白，但反之并不总是成立。
