Guinand M, Vacheron M J, Michel G, Tipper D J
J Bacteriol. 1979 Apr;138(1):126-32. doi: 10.1128/jb.138.1.126-132.1979.
The level of three peptidoglycan hydrolases was determined in the mother cell compartment and forespores of Bacillus sphaericus. Vegetative and sporulating cells contained in LD-carboxypeptidase active only on the vegetative cell wall peptidoglycan, and we have previously shown that sporulation is accompanied by the production of two new enzymes active only on the spore cortex peptidoglycan. These gamma-D-glutamyl-meso-diaminopimelate endopeptidase and a meso-diaminopimelate-D-alanine dipeptidase. The LD-carboxypeptidase activity appeared to be located in the membranes of both the mother cells and forespores. Endopeptidase activity was located in the integument fraction of the forespores, and the dipeptidase activity was only found in the forespore cytoplasm. These different locations comply with the probable different functions of these enzymes.
测定了球形芽孢杆菌母细胞区室和前芽孢中三种肽聚糖水解酶的水平。营养细胞和芽孢形成细胞中含有仅对营养细胞壁肽聚糖有活性的LD - 羧肽酶,并且我们之前已经表明,芽孢形成伴随着两种仅对芽孢皮层肽聚糖有活性的新酶的产生。这两种酶分别是γ - D - 谷氨酰 - 内消旋二氨基庚二酸内肽酶和内消旋二氨基庚二酸 - D - 丙氨酸二肽酶。LD - 羧肽酶活性似乎位于母细胞和前芽孢的膜中。内肽酶活性位于前芽孢的皮层部分,而二肽酶活性仅在前芽孢细胞质中发现。这些不同的定位符合这些酶可能具有的不同功能。