Racine F M, Vary J C
J Bacteriol. 1980 Sep;143(3):1208-14. doi: 10.1128/jb.143.3.1208-1214.1980.
Membranes from dormant and heat-activated spores of Bacillus megaterium QM B1551 were isolated and purified by gentle lysis procedures followed by differential and sucrose density gradient centrifugations. The purified membranes were enriched for inner membranes and were characterized by their density and content of proteins, phospholipids, enzymes, cytochromes, and carotenoids. These purified spore membranes could be used to investigate their role in the triggering of germination.
巨大芽孢杆菌QM B1551休眠孢子和热激活孢子的膜通过温和裂解程序，随后进行差速离心和蔗糖密度梯度离心进行分离和纯化。纯化后的膜富含内膜，并通过其密度以及蛋白质、磷脂、酶、细胞色素和类胡萝卜素的含量进行表征。这些纯化的芽孢膜可用于研究它们在萌发触发中的作用。
