Wang C S, Raper J R
Proc Natl Acad Sci U S A. 1970 Jul;66(3):882-9. doi: 10.1073/pnas.66.3.882.
Isozymes of several different classes of enzymes in partially purified protein extracts of five strains of Schizophyllum commune, isogenic except for genes controlling sexual morphogenesis, were separated on polyacrylamide gel by disc electrophoresis. After staining, isozyme patterns were compared on the bases of the presence or absence, electrophoretic mobility (R(f) values), and relative activities of specific isozymes. Differences in isozyme patterns in 14 enzymes, i.e., NADH-dehydrogenase, NADPH-dehydrogenase, a number of NAD and NADP-dependent dehydrogenases, acid phosphatases, leucine aminopeptidase, and esterases, were correlated with the operation or inactivity of the A-and B-sequences of sexual morphogenesis. In only a single instance, i.e., phenolases, no marked differences could be correlated with sexual morphogenesis.
除了控制有性形态发生的基因外，五株裂褶菌的部分纯化蛋白质提取物中几种不同类型酶的同工酶，通过圆盘电泳在聚丙烯酰胺凝胶上进行分离。染色后，根据特定同工酶的存在与否、电泳迁移率（R(f)值）和相对活性对同工酶模式进行比较。14种酶，即NADH脱氢酶、NADPH脱氢酶、多种NAD和NADP依赖性脱氢酶、酸性磷酸酶、亮氨酸氨肽酶和酯酶的同工酶模式差异，与有性形态发生的A序列和B序列的作用或无活性相关。仅在一个实例中，即酚酶，没有明显差异与有性形态发生相关。
