Graebe J E
Science. 1967 Jul 7;157(3784):73-5. doi: 10.1126/science.157.3784.73.
A cell-free system consisting of a soluble fraction and plastid fragments from pea shoots incorporates 2-(14)C-mevalonate very actively into farnesol, squalene, geranylgeraniol, and other isoprenoids of different carbon-chain lengths. The products have different cofactor requirements, which makes it possible to channel the pathway into different products by varying the incubation mixture.
一种由豌豆苗的可溶性部分和质体片段组成的无细胞体系能非常活跃地将2-(14)C-甲羟戊酸掺入法尼醇、角鲨烯、香叶基香叶醇以及其他不同碳链长度的类异戊二烯中。这些产物有不同的辅因子需求,这使得通过改变孵育混合物将该途径导向不同产物成为可能。