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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Charlton J M, Treharne K J, Goodwin T W

Biochem J. 1967 Oct;105(1):205-12. doi: 10.1042/bj1050205.

Chloroplasts prepared by the non-aqueous technique will, after fragmentation by ultrasonic treatment, incorporate [2-(14)C]mevalonic acid into phytoene, the first C(40) compound formed in the biosynthetic sequence to coloured carotenoids. 2. With suspensions containing 3.5mg. of chlorophyll, the optimum amounts of cofactor required were ATP (10mumoles), magnesium chloride (20mumoles) and glutathione (20mumoles); neither NAD(+) nor NADP(+) was required. 3. Very small amounts of squalene are also formed and synthesis is stimulated by addition of NADH or NADPH. Phytoene synthesis was not affected by the presence of these cofactors and no lycopersene (the C(40) homologue of squalene) was detected. 4. The phytol side chain of chlorophyll is also labelled under these conditions. 5. Preparations of developing chloroplasts are more active than preparations of mature chloroplasts.

通过非水技术制备的叶绿体，经超声处理破碎后，会将[2-(14)C]甲羟戊酸掺入到八氢番茄红素中，八氢番茄红素是生物合成序列中形成有色类胡萝卜素的首个C(40)化合物。2. 对于含有3.5毫克叶绿素的悬浮液，所需辅因子的最佳量为ATP（10微摩尔）、氯化镁（20微摩尔）和谷胱甘肽（20微摩尔）；不需要NAD(+) 也不需要NADP(+)。3. 也会形成极少量的角鲨烯，添加NADH或NADPH会刺激其合成。八氢番茄红素的合成不受这些辅因子存在的影响，未检测到番茄红素（角鲨烯的C(40)同系物）。4. 在这些条件下，叶绿素的叶绿醇侧链也会被标记。5. 发育中的叶绿体的制剂比成熟叶绿体的制剂更具活性。

Charlton J M, Treharne K J, Goodwin T W

Biochem J. 1967 Oct;105(1):205-12. doi: 10.1042/bj1050205.

Chloroplasts prepared by the non-aqueous technique will, after fragmentation by ultrasonic treatment, incorporate [2-(14)C]mevalonic acid into phytoene, the first C(40) compound formed in the biosynthetic sequence to coloured carotenoids. 2. With suspensions containing 3.5mg. of chlorophyll, the optimum amounts of cofactor required were ATP (10mumoles), magnesium chloride (20mumoles) and glutathione (20mumoles); neither NAD(+) nor NADP(+) was required. 3. Very small amounts of squalene are also formed and synthesis is stimulated by addition of NADH or NADPH. Phytoene synthesis was not affected by the presence of these cofactors and no lycopersene (the C(40) homologue of squalene) was detected. 4. The phytol side chain of chlorophyll is also labelled under these conditions. 5. Preparations of developing chloroplasts are more active than preparations of mature chloroplasts.

通过非水技术制备的叶绿体，经超声处理破碎后，会将[2-(14)C]甲羟戊酸掺入到八氢番茄红素中，八氢番茄红素是生物合成序列中形成有色类胡萝卜素的首个C(40)化合物。2. 对于含有3.5毫克叶绿素的悬浮液，所需辅因子的最佳量为ATP（10微摩尔）、氯化镁（20微摩尔）和谷胱甘肽（20微摩尔）；不需要NAD(+) 也不需要NADP(+)。3. 也会形成极少量的角鲨烯，添加NADH或NADPH会刺激其合成。八氢番茄红素的合成不受这些辅因子存在的影响，未检测到番茄红素（角鲨烯的C(40)同系物）。4. 在这些条件下，叶绿素的叶绿醇侧链也会被标记。5. 发育中的叶绿体的制剂比成熟叶绿体的制剂更具活性。