谷氨酸脱氢酶的晶体学研究。初步晶体数据。

Crystallographic studies of glutamate dehydrogenase. Preliminary crystal data.

作者信息

Birktoft J J, Miake F, Banaszak L J, Frieden C

出版信息

J Biol Chem. 1979 Jun 10;254(11):4915-8.

Abstract

Native and pyridoxal phosphate modified rat liver glutamate dehydrogenase crystals have been obtained and used for a preliminary x-ray crystallographic analysis. The space group is P6222 (P6422) having unit cell dimensions a = b = 101 A, c = 724 A and gamma = 120 A. The unit cell contains 36 subunits (six hexameric molecules) of molecular weight 56,000 and there is one half-molecule, i.e. three subunits, in the asymmetric unit. Packing considerations suggest that the glutamate dehydrogenase molecule has the point group symmetry 32 and that each subunit can be represented as a particle with approximate dimensions of 45 x 45 x 60 A.

摘要

已获得天然的和磷酸吡哆醛修饰的大鼠肝脏谷氨酸脱氢酶晶体，并将其用于初步的X射线晶体学分析。空间群为P6222（P6422），晶胞参数为a = b = 101 Å，c = 724 Å，γ = 120 Å。晶胞包含36个分子量为56,000的亚基（六个六聚体分子），不对称单位中有半个分子，即三个亚基。堆积分析表明，谷氨酸脱氢酶分子具有点群对称性32，每个亚基可表示为尺寸约为45×45×60 Å的颗粒。