Fallon R J, Cox R P, Ghosh N K
J Cell Physiol. 1979 Mar;98(3):613-8. doi: 10.1002/jcp.1040980319.
The growth of cervical carcinoma cell (HeLa) cultures in a hyperosmolar environment stimulates increased production of the onco-developmental peptides human choriogonadotropin (hCG) and follitropin (FSH). This effect was observed in two sublines examined in this study, HeLa65 and HeLa71. hCG and FSH were measured by radioimmunoassay using antiserum against the beta-subunit of the hormone dimer, thus insuring immunochemical specificity, The amounts of hCG and FSH produced by HeLa65 and HeLa71 cells cultured in hyperosmolar medium were 2- to 50-fold higher than corresponding hormone levels in basal cultures. Synthesis of gonadotropins depended on concentration and duration of exposure to hyperosmolar medium. Levels of culture medium osmolality effective in inducing hormone production also inhibit the incorporation of 14C-thymidine into acid-insoluble macromolecules. Hyperosmolality thus stimulates the ectopic production of gonadotropic hormones while retarding cellular growth and nucleic acid synthesis.
在高渗环境中培养宫颈癌细胞（HeLa）会刺激促癌发育肽人绒毛膜促性腺激素（hCG）和促卵泡激素（FSH）的产量增加。在本研究中检测的两个亚系HeLa65和HeLa71中观察到了这种效应。使用针对激素二聚体β亚基的抗血清通过放射免疫测定法测量hCG和FSH，从而确保免疫化学特异性。在高渗培养基中培养的HeLa65和HeLa71细胞产生的hCG和FSH量比基础培养物中的相应激素水平高2至50倍。促性腺激素的合成取决于暴露于高渗培养基的浓度和持续时间。有效诱导激素产生的培养基渗透压水平也会抑制14C-胸苷掺入酸不溶性大分子中。因此，高渗刺激促性腺激素的异位产生，同时延缓细胞生长和核酸合成。
