豚鼠肠黏膜中的脂肪酸合成
Fatty acid synthesis in intestinal mucosa of guinea pig.
作者信息
Tame M J, Kils R
出版信息
Biochem J. 1967 Nov;105(2):709-16. doi: 10.1042/bj1050709.
Abstract
- Acetate-CoA ligase, acetyl-CoA-carbon dioxide ligase and fatty acid synthetase were shown to be present in particle-free fractions of guinea-pig intestinal mucosa. 2. Each of these enzymes was partially purified by ammonium sulphate precipitation from the particle-free supernatant. 3. The incorporation of acetate and citrate into fatty acid was measured. 4. Gas-liquid radiochromatography was used to investigate the pattern of fatty acids synthesized. 5. The rate-limiting step in fatty acid synthesis was shown to be acetyl-CoA-carbon dioxide ligase.
摘要
- 已证明乙酸辅酶A连接酶、乙酰辅酶A-二氧化碳连接酶和脂肪酸合成酶存在于豚鼠肠黏膜的无颗粒组分中。2. 这些酶中的每一种都通过硫酸铵沉淀从无颗粒上清液中进行了部分纯化。3. 测定了乙酸和柠檬酸掺入脂肪酸的情况。4. 用气液放射性色谱法研究了合成脂肪酸的模式。5. 已表明脂肪酸合成中的限速步骤是乙酰辅酶A-二氧化碳连接酶。
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