马抗血清补体结合特性的研究。
A study of the complement fixing properties of equine antisera.
作者信息
Deutsch G, Amiraian K
出版信息
Immunology. 1968 Nov;15(5):623-32.
Buffers of low ionic strength enhanced the ability of both aged and recently prepared equine anti-pneumococcus sera to fix complement. Fixation physiological ionic strength, however, depended upon the age of the antisera. Sera 25–35 years old were not capable of fixing complement at physiological ionic strength, while antisera prepared and tested within 2 years fixed complement at this ionic strength. Heating the antisera at 56° for varying periods of time resulted in loss of ability to fix complement. Low ionic strength buffer partially obviated this loss. γG and γM immunoglobulin fractions were prepared from these anti-pneumococcus sera. The γG immunoglobulin fractions obtained did not fix complement. However, the complement-fixing properties of the γM immunoglobulins reflected those of the whole antiserum.
低离子强度的缓冲液增强了陈旧的和新制备的马抗肺炎球菌血清补体结合能力。然而，在生理离子强度下的补体结合情况取决于抗血清的年龄。25至35岁的血清在生理离子强度下不能结合补体，而在2年内制备和测试的抗血清在该离子强度下能结合补体。将抗血清在56℃加热不同时间导致补体结合能力丧失。低离子强度缓冲液部分消除了这种丧失。从这些抗肺炎球菌血清中制备了γG和γM免疫球蛋白组分。所获得的γG免疫球蛋白组分不能结合补体。然而，γM免疫球蛋白的补体结合特性反映了整个抗血清的特性。
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