Dacheux J L, O'Shea T, Paquignon M
J Reprod Fertil. 1979 Mar;55(2):287-96. doi: 10.1530/jrf.0.0550287.
Spermatozoa were collected from the rete testis of conscious boars, from the cauda epididymidis by retro-flushing, and by ejaculation. Testicular spermatozoa showed no progressive motility, and that of ejaculated was greater than that of epididymal spermatozoa. Glycolysis and respiration of testicular spermatozoa, while lower than that of the more mature cells, were only slightly affected by the incubation conditions. Epididymal spermatozoa converted 83% of the glucose they utilized to CO2 or lactate, but testicular cells converted only 35% to these metabolites. Synthesis of lipid was greatest by testicular spermatozoa. With the more mature cells hyperosmolar conditions depressed CO2 production, but increased lactate production, and these changes were greater for ejaculated than for epididymal spermatozoa. Glycolysis plus respiration of these cells was related to their motility. These results were interpreted as showing increasing motility, glycolysis and respiration with maturation, but also decreased synthetic capacity and increased sensitivity to the environment.
从清醒公猪的睾丸网、通过逆向冲洗从附睾尾部以及通过射精采集精子。睾丸精子没有表现出前进运动能力，而射精精子的运动能力大于附睾精子。睾丸精子的糖酵解和呼吸作用虽然低于更成熟细胞的水平，但仅受到孵育条件的轻微影响。附睾精子将其利用的葡萄糖的83%转化为二氧化碳或乳酸，而睾丸细胞仅将35%转化为这些代谢产物。睾丸精子的脂质合成最为旺盛。对于更成熟的细胞，高渗条件会抑制二氧化碳的产生，但会增加乳酸的产生，并且这些变化在射精精子中比在附睾精子中更大。这些细胞的糖酵解加呼吸作用与其运动能力相关。这些结果被解释为表明随着成熟，运动能力、糖酵解和呼吸作用增加，但合成能力下降且对环境的敏感性增加。
