用于监测微生物连续培养中还原型烟酰胺核苷酸水平变化的荧光技术。
Fluorimetric technique for monitoring changes in the level of reduced nicotinamide nucleotides in continuous cultures of microorganisms.
作者信息
Harrison D E, Chance B
出版信息
Appl Microbiol. 1970 Mar;19(3):446-50. doi: 10.1128/am.19.3.446-450.1970.
Abstract
A metabolite fluorimeter was modified to monitor nicotinamide nucleotides in cells growing in a chemostat culture. By illuminating and collecting fluorescence over a relatively large area of the side of the culture vessel, the noise level was kept low even in highly turbulent cultures. Calibration of the system was by enzymatic assay of samples. Changes in nicotinamide nucleotide of concentration less than 0.1 nmoles/ml could be detected [i.e., less than 10% aerobic/anaerobic response for a cell concentration of 5 mg (dry wt) per ml].
摘要
对一台代谢物荧光计进行了改装，以监测在恒化器培养中生长的细胞中的烟酰胺核苷酸。通过在培养容器侧面相对较大的区域上照射并收集荧光，即使在高度湍流的培养物中，噪声水平也能保持较低。该系统通过对样品进行酶促测定来校准。能够检测到烟酰胺核苷酸浓度小于0.1纳摩尔/毫升的变化[即，对于每毫升5毫克（干重）的细胞浓度，有氧/无氧响应小于10%]。
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