He Miao, Chen Mulan, Liu Mingxue, Dong Faqin, Wei Hongfu, Wang Danni
Life Science and Engineering College, Southwest University of Science and Technology Mianyang 621010 China
National Co-innovation Center for Nuclear Waste Disposal and Environmental Safety, Southwest University of Science and Technology Mianyang 621010 Sichuan China.
RSC Adv. 2019 Jul 25;9(40):22957-22965. doi: 10.1039/c9ra04066h. eCollection 2019 Jul 23.
Some microorganisms can utilize photoelectrons and electrode electrons. Exogenous electrons generate enough energy for growth, and electron shuttles may accelerate this process. This research data supported photoelectron-responsive microorganism was effected by the growth metabolism due to bias and electron shuttle riboflavin (RF) with an adaptive screening voltage under oligotrophic conditions. A slight change was observed in the redox property of RF. RF played the role of an electron shuttle. Microbial extracellular metabolites could bind additional nicotinamide adenine dinucleotide (NADH) species with RF. The intracellular protein content in the group of RF-Bias was 1.94, 1.93 and 4.02 times higher than those in the RF, bias and control groups, respectively, while the corresponding intracellular contents of humus were 1.10, 0.93 and 1.42 times higher. The content of CoA in RF-Bias, RF and bias increased to 116.0%, 108.5% and 103.8%, respectively. The organic acids of the RF-Bias group in the Krebs cycle are more advanced than those of other groups. Overall, in the Krebs cycle, RF and bias facilitated the growth and metabolism of . Finally, a mechanism was proposed, showing that the electron transfer chain and the Krebs cycle are stimulated by RF and bias.
一些微生物可以利用光电子和电极电子。外源电子产生足够的能量用于生长，电子穿梭体可能会加速这一过程。该研究数据表明，在贫营养条件下，具有自适应筛选电压的光电子响应微生物受到偏压和电子穿梭体核黄素（RF）的影响，其生长代谢发生了变化。观察到RF的氧化还原性质有轻微变化。RF起到了电子穿梭体的作用。微生物细胞外代谢产物可以使额外的烟酰胺腺嘌呤二核苷酸（NADH）物种与RF结合。RF-偏压组的细胞内蛋白质含量分别比RF组、偏压组和对照组高1.94倍、1.93倍和4.02倍，而腐殖质的相应细胞内含量分别高1.10倍、0.93倍和1.42倍。RF-偏压组、RF组和偏压组中辅酶A的含量分别增加到116.0%、108.5%和103.8%。RF-偏压组在三羧酸循环中的有机酸比其他组更高级。总体而言，在三羧酸循环中，RF和偏压促进了……的生长和代谢。最后，提出了一种机制，表明电子传递链和三羧酸循环受到RF和偏压的刺激。
