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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Sweetman A J, Griffiths D E

Biochem J. 1971 Jan;121(1):125-30. doi: 10.1042/bj1210125.

Small particles from Escherichia coli catalyse an ATP-dependent reduction of NADP(+) by NADH. 2. The reaction was stimulated by Mg(2+) and only ATP and ITP could serve as energy donors. 3. Studies of the stoicheiometry of the reaction indicate that 1-2mol of ATP are utilized/mol of NADH produced. 4. The reaction is inhibited by uncoupling agents such as 2,4-dinitrophenol (200mum) and tetrachlorotrifluorobenzimidazole (2mum) but oligomycin does not inhibit. 5. The reaction is inhibited by relatively high concentrations (200mum) of piericidin A.

来自大肠杆菌的小颗粒催化NADH对NADP(+)的ATP依赖性还原反应。2. 该反应受Mg(2+)刺激，只有ATP和ITP可作为能量供体。3. 对该反应化学计量学的研究表明，每产生1摩尔NADH会消耗1 - 2摩尔ATP。4. 该反应受解偶联剂如2,4 - 二硝基苯酚（200μM）和四氯三氟苯并咪唑（2μM）抑制，但寡霉素无抑制作用。5. 该反应受相对高浓度（200μM）的杀稻瘟菌素A抑制。

Sweetman A J, Griffiths D E

Biochem J. 1971 Jan;121(1):125-30. doi: 10.1042/bj1210125.

Small particles from Escherichia coli catalyse an ATP-dependent reduction of NADP(+) by NADH. 2. The reaction was stimulated by Mg(2+) and only ATP and ITP could serve as energy donors. 3. Studies of the stoicheiometry of the reaction indicate that 1-2mol of ATP are utilized/mol of NADH produced. 4. The reaction is inhibited by uncoupling agents such as 2,4-dinitrophenol (200mum) and tetrachlorotrifluorobenzimidazole (2mum) but oligomycin does not inhibit. 5. The reaction is inhibited by relatively high concentrations (200mum) of piericidin A.

来自大肠杆菌的小颗粒催化NADH对NADP(+)的ATP依赖性还原反应。2. 该反应受Mg(2+)刺激，只有ATP和ITP可作为能量供体。3. 对该反应化学计量学的研究表明，每产生1摩尔NADH会消耗1 - 2摩尔ATP。4. 该反应受解偶联剂如2,4 - 二硝基苯酚（200μM）和四氯三氟苯并咪唑（2μM）抑制，但寡霉素无抑制作用。5. 该反应受相对高浓度（200μM）的杀稻瘟菌素A抑制。