Aleem M I
J Bacteriol. 1966 Feb;91(2):729-36. doi: 10.1128/jb.91.2.729-736.1966.
Aleem, M. I. H. (Research Institute for Advanced Studies, Baltimore, Md.). Generation of reducing power in chemosynthesis. III. Energy-linked reduction of pyridine nucleotides in Thiobacillus novellus. J. Bacteriol. 91:729-736. 1966.-Cell-free extracts from Thiobacillus novellus. catalyzed an adenosine triphosphate (ATP)-dependent reduction of pyridine nucleotides anaerobically, or aerobically when the respiratory chain was inhibited by azide. The exogenous electron donor employed for the reduction of nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate (NADP) was thiosulfate, formate, or mammalian ferrocytochrome c. In the latter case, the oxidation of ferrocytochrome c was observed with the concomitant reduction of the pyridine nucleotide. Values calculated for the molar ratios of ATP utilized-NADP reduced and of cytochrome c oxidized-NADP reduced were 1:1 and 2:1, respectively. The energy-dependent reduction of the pyridine nucleotides was inhibited by Atabrine or amytal and by low concentrations of the uncouplers of oxidative phosphorylation such as 2,4-dinitrophenol and carbonyl cyanide m-chlorophenyl hydrazone. Evidence is presented showing that the reduced pyridine nucleotides are essential for providing the reducing power for the energy-dependent reduction of carbon dioxide in T. novellus.
阿利姆，M. I. H.（马里兰州巴尔的摩高级研究学会）。化学合成中还原力的产生。III. 新型硫杆菌中吡啶核苷酸的能量偶联还原。《细菌学杂志》91:729 - 736。1966年。——新型硫杆菌的无细胞提取物在无氧条件下，或当呼吸链被叠氮化物抑制时在有氧条件下，催化了三磷酸腺苷（ATP）依赖的吡啶核苷酸还原反应。用于还原烟酰胺腺嘌呤二核苷酸或烟酰胺腺嘌呤二核苷酸磷酸（NADP）的外源电子供体是硫代硫酸盐、甲酸盐或哺乳动物亚铁细胞色素c。在后一种情况下，观察到亚铁细胞色素c被氧化，同时吡啶核苷酸被还原。计算得出的ATP利用量与NADP还原量的摩尔比以及细胞色素c氧化量与NADP还原量的摩尔比分别为1:1和2:1。吡啶核苷酸的能量依赖还原反应受到阿的平或戊巴比妥以及低浓度的氧化磷酸化解偶联剂（如2,4 -二硝基苯酚和羰基氰化物间氯苯腙）的抑制。有证据表明，还原型吡啶核苷酸对于为新型硫杆菌中能量依赖的二氧化碳还原提供还原力至关重要。
