核糖体的光亲和标记 - Suppr | 超能文献

Photoaffinity labeling of ribosomes.

作者信息

Cooperman B S, Grant P G, Goldman R A, Luddy M A, Minnella A, Nicholson A W, Strycharz W A

出版信息

Methods Enzymol. 1979;59:796-815. doi: 10.1016/0076-6879(79)59126-5.

DOI:10.1016/0076-6879(79)59126-5

Abstract

摘要

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Photoaffinity labeling of ribosomes.核糖体的光亲和标记

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Photoaffinity labeling of Escherichia coli ribosomes by an aryl azide analogue of puromycin. On the identification of the major covalently labeled ribosomal proteins and on the mechanism of photoincorporation.用嘌呤霉素的芳基叠氮类似物对大肠杆菌核糖体进行光亲和标记。关于主要共价标记核糖体蛋白的鉴定及光掺入机制。

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3

About the specificity of photoinduced affinity labeling of Escherichia coli ribosomes by dihydrorosaramicin, a macrolide related to erythromycin.

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Photoaffinity labeling of rat liver ribosomes by N-(2-nitro-4-azidobenzoyl)puromycin.用N-(2-硝基-4-叠氮苯甲酰)嘌呤霉素对大鼠肝脏核糖体进行光亲和标记。

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Some methods using protection from chemical and enzymic modification to determine protein-protein relationships in ribosomes.一些利用防止化学和酶促修饰的方法来确定核糖体中蛋白质-蛋白质关系。

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Puromycin photoaffinity labels small- and large-subunit proteins at the A site of the Drosophila ribosome.嘌呤霉素光亲和标记果蝇核糖体A位点的小亚基和大亚基蛋白。

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Photoinduced affinity labeling of the Escherichia coli ribosome puromycin site.大肠杆菌核糖体嘌呤霉素位点的光诱导亲和标记

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Immunoelectron microscopic localization of the site of photo-induced affinity labeling of the small ribosomal subunit with puromycin.用嘌呤霉素对小核糖体亚基进行光诱导亲和标记位点的免疫电子显微镜定位。

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9

Cross-linking of ribosomes using 2-iminothiolane (methyl 4-mercaptobutyrimidate) and identification of cross-linked proteins by diagonal polyacrylamide/sodium dodecyl sulfate gel electrophoresis.使用2-亚氨基硫杂环戊烷（4-巯基丁酸甲酯）对核糖体进行交联，并通过对角线聚丙烯酰胺/十二烷基硫酸钠凝胶电泳鉴定交联蛋白。

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Photoaffinity labeling of Escherichia coli ribosomes by an aryl azide analogue of puromycin. Evidence for the functional site specificity of labeling.用嘌呤霉素的芳基叠氮类似物对大肠杆菌核糖体进行光亲和标记。标记功能位点特异性的证据。

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引用本文的文献

1

Immunoelectron microscopic localization of the site of photo-induced affinity labeling of the small ribosomal subunit with puromycin.用嘌呤霉素对小核糖体亚基进行光诱导亲和标记位点的免疫电子显微镜定位。

Proc Natl Acad Sci U S A. 1980 Feb;77(2):890-4. doi: 10.1073/pnas.77.2.890.