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脑中4-羟基-3-甲氧基苯乙二醇硫酸盐的荧光测定

Fluorometric estimation of 4-hydroxy-3-methoxyphenylethyleneglycol sulphate in brain.

作者信息

Meek J L, Neff N H

出版信息

Br J Pharmacol. 1972 Jul;45(3):435-41. doi: 10.1111/j.1476-5381.1972.tb08099.x.

DOI:10.1111/j.1476-5381.1972.tb08099.x
PMID:4403685
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1666152/
Abstract
  1. A simple, sensitive, and selective method was developed for the fluorimetric estimation of 4-hydroxy-3-methoxyphenylethyleneglycol sulphate (MOPEG-SO(4)) in rat brain.2. MOPEG-SO(4) was isolated on a DEAE Sephadex column. Fluorescence was measured at 465 nm (excitation at 320 nm) after heating the column eluate with acid and reheating after adding ethylene diamine.3. The concentration in brain of MOPEG-SO(4) increased after blockade of its transport from brain by treatment with probenecid and decreased after inhibiting its synthesis by treatment with alpha-methyltyrosine or pargyline.4. Our studies suggest that measurement in changes in the rate of formation of MOPEG-SO(4) may be a useful index of noradrenaline turnover.
摘要

  1. 开发了一种简单、灵敏且具选择性的方法，用于荧光法测定大鼠脑中的4-羟基-3-甲氧基苯乙二醇硫酸盐（MOPEG-SO(4)）。

  2. MOPEG-SO(4)在DEAE葡聚糖柱上进行分离。用酸加热柱洗脱液并在加入乙二胺后再次加热后，于465nm处（激发波长为320nm）测量荧光。

  3. 用丙磺舒处理阻断其从脑内的转运后，脑中MOPEG-SO(4)的浓度升高；用α-甲基酪氨酸或帕吉林处理抑制其合成后，浓度降低。

  4. 我们的研究表明，测定MOPEG-SO(4)生成速率的变化可能是去甲肾上腺素周转的一个有用指标。

相似文献

1
Fluorometric estimation of 4-hydroxy-3-methoxyphenylethyleneglycol sulphate in brain.脑中4-羟基-3-甲氧基苯乙二醇硫酸盐的荧光测定
Br J Pharmacol. 1972 Jul;45(3):435-41. doi: 10.1111/j.1476-5381.1972.tb08099.x.
2
Failure to detect 5-hydroxytryptamine-O-sulphate in normal rat brain and after monoamine oxidase inhibition.在正常大鼠脑内及单胺氧化酶抑制后未能检测到5-羟色胺-O-硫酸盐。
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3
Short and long-term effects of reserpine on the concentration of 1-(4-hydroxy-3-methoxyphenyl)-ethane-1,2-diol (MOPEG-SO4) in the brain of the rat.利血平对大鼠脑内1-(4-羟基-3-甲氧基苯基)-乙烷-1,2-二醇（MOPEG-SO4）浓度的短期和长期影响。
Br J Pharmacol. 1979 Apr;65(4):573-8. doi: 10.1111/j.1476-5381.1979.tb07866.x.
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The rate of formation of 3-methoxy-4-hydroxyphenylethyleneglycol sulfate in brain as an estimate of the rate of formation of norepinephrine.脑中3-甲氧基-4-羟基苯乙二醇硫酸盐的生成速率，作为去甲肾上腺素生成速率的一种估计。
J Pharmacol Exp Ther. 1973 Mar;184(3):570-5.
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Glycol sulphate ester formation from ( 14 C)noradrenaline in brain and the influence of a COMT inhibitor.大脑中由（14C）去甲肾上腺素形成硫酸甘醇酯以及儿茶酚-O-甲基转移酶抑制剂的影响。
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Effects of acute cocaine treatment on the turnover of 5-hydroxytryptamine in the rat brain.急性可卡因处理对大鼠脑内5-羟色胺更新率的影响。
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The effect of trauma on the activity of central noradrenergic neurones.创伤对中枢去甲肾上腺素能神经元活性的影响。
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Effect of prazosin on norepinephrine concentration and turnover in rat brain and heart.哌唑嗪对大鼠脑和心脏中去甲肾上腺素浓度及周转率的影响。
Arch Int Pharmacodyn Ther. 1978 Jan;231(1):30-41.

引用本文的文献

1
Characteristics of enzymes forming 3-methoxy-4-hydroxyphenylethyleneglycol (MOPEG) in brain.脑内形成 3-甲氧基-4-羟苯乙二醇（MOPEG）的酶的特性。
Neurochem Res. 1976 Oct;1(5):525-40. doi: 10.1007/BF00964213.
2
Proceedings of the British Pharmacological Society. 17th-19th December, 1979: University of London (Institute of Education).英国药理学会会议记录。1979年12月17日至19日：伦敦大学（教育学院）
Br J Pharmacol. 1980 Jun;69(2):265P-346P.
3
Effect of low-dose lithium administration and subsequent withdrawal on biogenic amines in rat brain.低剂量锂给药及随后停药对大鼠脑内生物胺的影响。
Br J Pharmacol. 1980;71(2):601-7. doi: 10.1111/j.1476-5381.1980.tb10979.x.
4
Effect of tyrosine on brain catecholamine turnover in reserpine-treated rats.酪氨酸对利血平处理大鼠脑内儿茶酚胺周转率的影响。
J Neural Transm. 1982;53(2-3):101-8. doi: 10.1007/BF01243401.
5
Effect of a reversible and selective MAO-A inhibitor (cimoxatone) on diurnal variation in plasma prolactin level in man.可逆性选择性单胺氧化酶-A抑制剂（西莫沙酮）对人体血浆催乳素水平昼夜变化的影响。
Eur J Clin Pharmacol. 1984;26(1):71-7. doi: 10.1007/BF00546712.
6
Effects of thyroid status on presynaptic alpha 2-adrenoceptor function and beta-adrenoceptor binding in the rat brain.甲状腺状态对大鼠脑内突触前α2-肾上腺素能受体功能及β-肾上腺素能受体结合的影响。
J Neural Transm. 1984;59(1):43-55. doi: 10.1007/BF01249877.
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Diazepam potentiates the effect of neuroleptics on behavioural activity as well as dopamine and norepinephrine turnover: Do benzodiazepines have antipsychotic potency?地西泮可增强抗精神病药物对行为活动以及多巴胺和去甲肾上腺素代谢的作用：苯二氮䓬类药物具有抗精神病效力吗？
J Neural Transm. 1983;56(2-3):127-38. doi: 10.1007/BF01243272.
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A study of the sensitivity of rat brain alpha 2-adrenoceptors during chronic antidepressant treatments.一项关于慢性抗抑郁治疗期间大鼠脑α2-肾上腺素能受体敏感性的研究。
Naunyn Schmiedebergs Arch Pharmacol. 1982 Aug;320(2):90-6. doi: 10.1007/BF00506306.
9
Effects of CGP 11305 A, a new reversible and selective inhibitor of MAO A, on biogenic amine levels and metabolism in the rat brain.新型单胺氧化酶A可逆性选择性抑制剂CGP 11305 A对大鼠脑内生物胺水平及代谢的影响
Naunyn Schmiedebergs Arch Pharmacol. 1983 Sep;324(1):20-6. doi: 10.1007/BF00647833.
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Effects of acute administration of cyclazocine on the metabolism of biogenic amines in different regions of rat brain.环唑辛急性给药对大鼠脑不同区域生物胺代谢的影响。
Psychopharmacology (Berl). 1981;75(1):79-83. doi: 10.1007/BF00433507.

本文引用的文献

1
Determination of urinary 3-methoxy-4-hydroxyphenyl-ethylene glycol by gas-liquid chromatography and electron capture detection.采用气液色谱法和电子捕获检测法测定尿中3-甲氧基-4-羟基苯乙二醇。
Clin Chim Acta. 1967 Jun;16(3):403-8. doi: 10.1016/0009-8981(67)90306-3.
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Regional studies of catecholamines in the rat brain. I. The disposition of [3H]norepinephrine, [3H]dopamine and [3H]dopa in various regions of the brain.大鼠脑中儿茶酚胺的区域研究。I. [3H]去甲肾上腺素、[3H]多巴胺和[3H]多巴在脑不同区域的分布。
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Glycol metabolites of noradrenaline in brain tissue.脑组织中去甲肾上腺素的糖代谢产物
Br J Pharmacol. 1969 Jul;36(3):523-34. doi: 10.1111/j.1476-5381.1969.tb08008.x.
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Metabolism of normetanephrine-H3 in rat brain--identification of conjugated 3-methoxy-4-hydrophenylglycol as the major metabolite.大鼠脑中去甲变肾上腺素 - H3的代谢——鉴定结合型3 - 甲氧基 - 4 - 羟基苯乙二醇为主要代谢产物。
Biochem Pharmacol. 1968 Feb;17(2):247-54. doi: 10.1016/0006-2952(68)90330-4.
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Effect of intravenously administered probenecid in humans on the levels of 5-hydroxyindoleacetic acid, homovanillic acid and 3-methoxy-4-hydroxy-phenylglycol in cerebrospinal fluid.静脉注射丙磺舒对人体脑脊液中5-羟吲哚乙酸、高香草酸和3-甲氧基-4-羟基苯乙二醇水平的影响。
Biochem Pharmacol. 1971 Mar;20(3):659-68. doi: 10.1016/0006-2952(71)90152-3.
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3-methoxy-4-hydroxyphenylglycol sulfate in brain and cerebrospinal fluid.大脑和脑脊液中的3-甲氧基-4-羟基苯乙二醇硫酸盐
Biochem Pharmacol. 1968 Sep;17(9):2006-8. doi: 10.1016/0006-2952(68)90119-6.
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3 -Methoxy- 4 -hydroxyphenylethylene glycol in human cerebrospinal fluid.人脑脊液中的3-甲氧基-4-羟基苯乙二醇
Clin Chim Acta. 1971 Nov;35(1):145-50. doi: 10.1016/0009-8981(71)90305-6.