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脑组织中去甲肾上腺素的糖代谢产物

Glycol metabolites of noradrenaline in brain tissue.

作者信息

Sharman D F

出版信息

Br J Pharmacol. 1969 Jul;36(3):523-34. doi: 10.1111/j.1476-5381.1969.tb08008.x.

DOI:10.1111/j.1476-5381.1969.tb08008.x
PMID:5789807
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1703619/
Abstract
  1. A gas chromatographic method using electron capture detection is described for the estimation of those catecholamines and their metabolites which possess one or two free alcoholic hydroxyl groups. It is based on acetylation of phenolic hydroxyl and primary and secondary amino groups in aqueous solution, extraction into a water immiscible solvent and esterification of alcoholic hydroxyl groups with heptafluorobutyric anhydride.2. The method has been successfully applied to the detection and estimation of free 4-hydroxy-3-methoxyphenylethylene glycol (MOPEG) and free 3,4-dihydroxyphenylethylene glycol (DOPEG) in hypothalamic brain tissue.3. Although it was possible to measure noradrenaline and normetanephrine by the same method in pure solution, crude tissue extracts could not be used, despite their suitability for estimating the glycol metabolites.
摘要

  1. 描述了一种使用电子捕获检测的气相色谱法，用于估算那些具有一个或两个游离醇羟基的儿茶酚胺及其代谢产物。该方法基于酚羟基以及水溶液中的伯氨基和仲氨基的乙酰化反应，萃取到与水不混溶的溶剂中，并使用七氟丁酸酐将醇羟基酯化。

  2. 该方法已成功应用于下丘脑脑组织中游离4-羟基-3-甲氧基苯乙二醇（MOPEG）和游离3,4-二羟基苯乙二醇（DOPEG）的检测和估算。

  3. 尽管通过相同方法在纯溶液中可以测量去甲肾上腺素和去甲变肾上腺素，但粗制组织提取物不能使用，尽管它们适用于估算二醇代谢产物。

相似文献

1
Glycol metabolites of noradrenaline in brain tissue.脑组织中去甲肾上腺素的糖代谢产物
Br J Pharmacol. 1969 Jul;36(3):523-34. doi: 10.1111/j.1476-5381.1969.tb08008.x.
2
Studies on the metabolism of catecholamines in the central nervous system of the mouse.小鼠中枢神经系统中儿茶酚胺代谢的研究。
Br J Pharmacol. 1974 Jun;51(2):187-95. doi: 10.1111/j.1476-5381.1974.tb09646.x.
3
Determination of normetanephrine, 3,4-dihydroxyphenylethyleneglycol (free and total), and 3-methoxy-4-hydroxyphenylethyleneglycol (free and total) in rat brain by high-performance liquid chromatography with electrochemical detection and effects of drugs on regional concentrations.采用高效液相色谱-电化学检测法测定大鼠脑中去甲变肾上腺素、3,4-二羟基苯乙二醇（游离型和总型）以及3-甲氧基-4-羟基苯乙二醇（游离型和总型），并研究药物对局部浓度的影响。
J Neurochem. 1984 Apr;42(4):934-42. doi: 10.1111/j.1471-4159.1984.tb12694.x.
4
A method for the assay of conjugated 3,4-dihydroxyphenylglycol, a major noradrenaline metabolite in the rat brain.一种用于测定大鼠脑中主要去甲肾上腺素代谢物——结合型3,4-二羟基苯乙二醇的方法。
J Neurochem. 1976 Nov;27(5):1211-7. doi: 10.1111/j.1471-4159.1976.tb00329.x.
5
Simultaneous measurement of plasma 4-hydroxy-3-methoxyphenylethylene glycol and 3,4-dihydroxyphenylethylene glycol by gas chromatography mass spectrometry.采用气相色谱-质谱联用技术同时测定血浆4-羟基-3-甲氧基苯乙二醇和3,4-二羟基苯乙二醇
Biomed Mass Spectrom. 1981 Jun;8(6):256-9. doi: 10.1002/bms.1200080606.
6
Intra- and extraneuronal formation of the two major noradrenaline metabolites in the cns of rats.大鼠中枢神经系统中两种主要去甲肾上腺素代谢产物在神经元内和神经元外的形成。
J Pharm Pharmacol. 1975 Jun;27(6):413-9. doi: 10.1111/j.2042-7158.1975.tb09470.x.
7
Norepinephrine metabolism in canine saphenous vein: prevalence of glycol metabolites.犬隐静脉中的去甲肾上腺素代谢：糖醇代谢产物的普遍性
Am J Physiol. 1978 Mar;234(3):H235-43. doi: 10.1152/ajpheart.1978.234.3.H235.
8
[Study of the metabolism of cerebral noradrenaline in depressed patients by the assay of plasma dihydroxyphenylethylene glycol].通过血浆二羟苯乙二醇测定法研究抑郁症患者脑中去甲肾上腺素的代谢
Presse Med. 1985 Jun 22;14(25):1363-6.
9
A method for the estimation of the catecholamines and their metabolites in brain tissue.一种用于估计脑组织中儿茶酚胺及其代谢物的方法。
Br J Pharmacol. 1970 Jan;38(1):56-71. doi: 10.1111/j.1476-5381.1970.tb10336.x.
10
Liquid chromatographic determination of free and conjugated 3-methoxy-4-hydroxyphenylethylene glycol with wide-ranging substances related to monoamine metabolism.液相色谱法测定游离和结合型3-甲氧基-4-羟基苯乙二醇以及与单胺代谢相关的多种物质。
Anal Biochem. 1985 Jun;147(2):441-50. doi: 10.1016/0003-2697(85)90295-7.

引用本文的文献

1
The involvement of noradrenergic transmission in the morphine-induced locomotor hyperactivity in mice withdrawn from repeated morphine treatment.去甲肾上腺素能传递在反复给予吗啡处理后停药的小鼠吗啡诱导的运动性活动亢进中的作用。
Br J Pharmacol. 1999 Apr;126(7):1609-19. doi: 10.1038/sj.bjp.0702485.
2
Proceedings: Studies on the glycol metabolites of noradrenaline in mouse brain.论文集：小鼠脑中去甲肾上腺素的糖代谢产物研究
Br J Pharmacol. 1972 Feb;44(2):340P-341P.
3
The effect of electrical stimulation of the locus coeruleus on the endogenous concentration of 4-hydroxy-3-methoxy-phenylethylene glycol in rat brain.蓝斑核的电刺激对大鼠脑内4-羟基-3-甲氧基苯乙二醇内源性浓度的影响。
Biochem J. 1972 Jul;128(3):85P-86P. doi: 10.1042/bj1280085pb.
4
Morphine analgesia and the bulbospinal noradrenergic system: increase in the concentration of normetanephrine in the spinal cord of the rat caused by analgesics.吗啡镇痛与延髓脊髓去甲肾上腺素能系统：镇痛药引起大鼠脊髓中去甲变肾上腺素浓度增加。
Br J Pharmacol. 1974 Dec;52(4):519-26. doi: 10.1111/j.1476-5381.1974.tb09719.x.
5
Fluorometric estimation of 4-hydroxy-3-methoxyphenylethyleneglycol sulphate in brain.脑中4-羟基-3-甲氧基苯乙二醇硫酸盐的荧光测定
Br J Pharmacol. 1972 Jul;45(3):435-41. doi: 10.1111/j.1476-5381.1972.tb08099.x.
6
Studies on the metabolism of catecholamines in the central nervous system of the mouse.小鼠中枢神经系统中儿茶酚胺代谢的研究。
Br J Pharmacol. 1974 Jun;51(2):187-95. doi: 10.1111/j.1476-5381.1974.tb09646.x.
7
Measurement of 3-methoxy-4-hydroxyphenylglycol (MHPG) in mouse brain by h.p.l.c. with electrochemical detection, as an index of noradrenaline utilisation and presynaptic alpha 2-adrenoceptor function.采用高效液相色谱法（h.p.l.c.）并结合电化学检测测定小鼠脑中3-甲氧基-4-羟基苯乙二醇（MHPG），以此作为去甲肾上腺素利用及突触前α2-肾上腺素能受体功能的指标。
Br J Pharmacol. 1989 Mar;96(3):547-56. doi: 10.1111/j.1476-5381.1989.tb11852.x.
8
Brain cortical tissue levels of noradrenaline and its glycol metabolites: effects of ischemia and postischemic administration of idazoxan.大脑皮质组织中去甲肾上腺素及其二醇代谢产物的水平：缺血及缺血后给予咪唑克生的影响
Exp Brain Res. 1992;90(3):551-6. doi: 10.1007/BF00230938.
9
Monoamine metabolites in cerebrospinal fluid in multiple sclerosis.多发性硬化症患者脑脊液中的单胺代谢产物
J Neurol Neurosurg Psychiatry. 1977 Aug;40(8):741-5. doi: 10.1136/jnnp.40.8.741.
10
A study of the formation and metabolic disposition of 3,4 dihydroxyphenylethyleneglycol in whole rat brain.大鼠全脑内3,4-二羟基苯乙二醇的形成及代谢处置研究
J Neural Transm. 1977;41(1):59-72. doi: 10.1007/BF01252965.

本文引用的文献

1
Methods of paper chromatography of steroids applicable to the study of steroids in mammalian blood and tissues.适用于研究哺乳动物血液和组织中类固醇的类固醇纸色谱法。
Biochem J. 1952 Jan;50(3):370-8. doi: 10.1042/bj0500370.
2
THE ESTIMATION OF SMALL QUANTITIES OF 3,4-DIHYDROXYPHENYLETHYLAMINE IN TISSUES.组织中少量3,4-二羟基苯乙胺的测定
Br J Pharmacol Chemother. 1965 Apr;24(2):538-48. doi: 10.1111/j.1476-5381.1965.tb01744.x.
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PRECISE ULTRAMICRO DETERMINATION OF STEROID HORMONES BY COMBINED TLC AND GLC ANALYSIS.采用薄层色谱法和气相色谱法联用对甾体激素进行精确超微量测定。
J Am Oil Chem Soc. 1965 Apr;42:283-90. doi: 10.1007/BF02540130.
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METABOLISM OF (H3)NOREPINEPHRINE IN THE RAT BRAIN.大鼠脑中（H3）去甲肾上腺素的代谢
J Neurochem. 1965 Jan;12:25-30. doi: 10.1111/j.1471-4159.1965.tb10247.x.
5
A MICOQUANTITATIVE TECHNIQUE FOR THE DETECTION OF SOME BIOLOGICALLY IMPORTANT AMINES BY GAS-LIQUID CHROMATOGRAPHY.一种通过气液色谱法检测某些具有重要生物学意义的胺类的微量定量技术。
Clin Chim Acta. 1964 Aug;10:193-5. doi: 10.1016/0009-8981(64)90166-4.
6
A fluorimetric method for the estimation of 4-hydroxy-3-methoxyphenylacetic acid (homovanillic acid) and its identification in brain tissue.一种用于估算4-羟基-3-甲氧基苯乙酸（高香草酸）及其在脑组织中鉴定的荧光法。
Br J Pharmacol Chemother. 1963 Feb;20(1):204-13. doi: 10.1111/j.1476-5381.1963.tb01310.x.
7
A method for the isolation and separation of catechol amines and their transformation products from biological media.一种从生物介质中分离和提纯儿茶酚胺及其转化产物的方法。
Anal Biochem. 1961 Aug;2:387-90. doi: 10.1016/0003-2697(61)90013-6.
8
The analysis of solutions of epinephrine and norepinephrine.肾上腺素与去甲肾上腺素溶液的分析
J Am Pharm Assoc Am Pharm Assoc. 1955 Aug;44(8):507-14. doi: 10.1002/jps.3030440815.
9
Determination of urinary 3-methoxy-4-hydroxyphenyl-ethylene glycol by gas-liquid chromatography and electron capture detection.采用气液色谱法和电子捕获检测法测定尿中3-甲氧基-4-羟基苯乙二醇。
Clin Chim Acta. 1967 Jun;16(3):403-8. doi: 10.1016/0009-8981(67)90306-3.
10
Gas chromatographic evidence for the presence of glycol metabolites of catecholamines in brain tissue.脑组织中儿茶酚胺糖代谢产物存在的气相色谱证据。
J Physiol. 1969 Jan;200(1):33P-5P.