Shah B G, Giroux A, Belonje B, Jones J D
Nutr Metab. 1979;23(4):275-85.
Three groups of 33 90-day-old female Sprague-Dawley rats were fed, ad libitum, the following diets for 2 weeks before breeding. Diet 1 (D1) contained 20% protein from casein, diet 2 (D2) had the same level of protein from Tower rapeseed (Brassica napus) protein concontrate (RPC) and diet 3 (D3) was the same as D2 with a zinc supplement (70 mg/l) in the drinking water. From each group 6 animals were killed before breeding and 5-9 animals were killed at 1 and 2 weeks of gestation and post-partum. From each rat, blood, thyroids, liver and femur were collected for the determination of zinc, copper, iron, manganese, calcium and magnesium. As a measure of the reproductive performance, body weight, number of pups in the uterus or delivered live or dead, and gestations days before parturition were recorded. The pups were examined for obvious deformities and also analysed for the above mineral elements by atomic absorption spectroscopy. In group D2, levels of zinc in maternal serum, liver, femur and in the pups were significantly lower than the comparable levels in the other two groups. The zinc supplemented RPC-fed group did not show the anorexia experienced by the unsupplemented group and there was neither a significant difference between reproductive performances of groups D1 and D3 nor was there any significant difference between the zinc levels determined. It was concluded that the toxic symptoms caused by RPC feeding was attributable to zinc deficiency probably caused by the high phytate level in the RPC.
将三组90日龄的雌性斯普拉格-道利大鼠在繁殖前自由采食以下日粮2周。日粮1（D1）含有20%的酪蛋白蛋白质，日粮2（D2）含有相同水平的来自塔油菜籽（甘蓝型油菜）浓缩蛋白（RPC）的蛋白质，日粮3（D3）与D2相同，但饮用水中添加了锌（70毫克/升）。每组在繁殖前处死6只动物，在妊娠1周和2周以及产后处死5 - 9只动物。从每只大鼠采集血液、甲状腺、肝脏和股骨，用于测定锌、铜、铁、锰、钙和镁。作为生殖性能的衡量指标，记录体重、子宫内活产或死产幼崽数量以及分娩前的妊娠天数。检查幼崽是否有明显畸形，并通过原子吸收光谱法分析上述矿物质元素。在D2组中，母体血清、肝脏、股骨以及幼崽体内的锌水平显著低于其他两组的相应水平。添加锌的RPC喂养组没有出现未添加锌组所经历的厌食情况，D1组和D3组的生殖性能之间没有显著差异，所测定的锌水平之间也没有显著差异。得出的结论是，RPC喂养引起的毒性症状可能归因于RPC中植酸水平高导致的锌缺乏。
