Page W J, Stock J J
J Bacteriol. 1974 Jul;119(1):44-9. doi: 10.1128/jb.119.1.44-49.1974.
Ethylenediamine-soluble glycoproteins were extracted from isolated Microsporum gypseum hyphal walls during sporulation and from spore coats before and after germination. This study was carried out to identify a sporulation-specific cell wall protein that possibly served as a substrate for the alkaline protease which initiated the macroconidial germination of this fungus. Analyses revealed that water-insoluble glycoprotein accounted for 10% of the ungerminated spore coat but only for 4 to 5% of the mycelial wall dry weight. This fraction was modified in its amino acid composition during sporulation, and it decreased in protein content during spore germination. Water-soluble glycoprotein, which accounted for approximately 3 to 3.5% of either the spore coat or mycelial wall dry weight, was of similar amino acid composition from both sources and did not decrease in protein content upon spore germination. The water-insoluble glycoprotein was found to be rich in leucine, aspartic acid, glycine, glutamic acid, and phenylalanine residues. The water-soluble glycoprotein was rich in proline, threonine, glycine, serine, glutamic acid, and alanine.
在孢子形成过程中从分离的石膏样小孢子菌菌丝壁中提取乙二胺可溶性糖蛋白，并在孢子萌发前后从孢子壁中提取。开展本研究以鉴定一种孢子形成特异性细胞壁蛋白，其可能作为启动该真菌大分生孢子萌发的碱性蛋白酶的底物。分析表明，水不溶性糖蛋白占未萌发孢子壁的10%，但仅占菌丝壁干重的4%至5%。该部分在孢子形成过程中其氨基酸组成发生改变，并且在孢子萌发过程中蛋白质含量降低。水溶性糖蛋白约占孢子壁或菌丝壁干重的3%至3.5%，来自这两种来源的水溶性糖蛋白具有相似的氨基酸组成，并且在孢子萌发时蛋白质含量不降低。发现水不溶性糖蛋白富含亮氨酸、天冬氨酸、甘氨酸、谷氨酸和苯丙氨酸残基。水溶性糖蛋白富含脯氨酸、苏氨酸、甘氨酸、丝氨酸、谷氨酸和丙氨酸。
