用固定化tRNA研究tRNA甲基化酶的作用机制。
The mechanism of action of tRNA methylases studied with immobilized tRNAs.
作者信息
Gambaryan A S, Morozov I A, Venkstern T V, Bayev A A
出版信息
Nucleic Acids Res. 1979 Mar;6(3):1001-11. doi: 10.1093/nar/6.3.1001.
Each of the individual tRNAs immobilized on aminohydroxybutyl-cellulose (ABC) through their oxidized 3'-terminal binds affinitively all methylases present in the enzyme extract irrespective of whether this tRNA will be involved in the following step of methylation or not. These data allow to suggest that (a) the formation of a methylase-tRNA complex and the catalytic act of methylation are indeed autonomous processes and (b) the first step of interaction between tRNAs and tRNA methylases is rather unspecific and consists in the recognition of the whole class of tRNA molecules.
通过其氧化的3'-末端固定在氨基羟基丁基纤维素（ABC）上的每一种单个转运RNA（tRNA），都能亲和地结合酶提取物中存在的所有甲基化酶，无论该tRNA是否会参与随后的甲基化步骤。这些数据表明：（a）甲基化酶 - tRNA复合物的形成和甲基化催化作用确实是自主过程；（b）tRNA与tRNA甲基化酶之间相互作用的第一步相当非特异性，在于识别整个tRNA分子类别。
Zotero 插件