关于tRNA甲基化酶与tRNA识别的机制
On the mechanism of tRNA methylase-tRNA recognition.
作者信息
Gambaryan A S, Venkstern T V, Bayev A A
出版信息
Nucleic Acids Res. 1976 Aug;3(8):2079-87. doi: 10.1093/nar/3.8.2079.
In order to further elucidate the mechanism of tRNA methylase-tRNA intreaction the methylation of some individual tRNAs separately and by pairs was performed. In conditions of tRNA excess the methylation rates of positionally analogous nucleotides in tRNA molecules are not summed up when two substrates are simultaneously present in the reaction mixture. The inhibitory action of yeast tRNASer, possessing m5c in position 29, on the methylation of C29 in other individual tRNAs was shown. Yeast tRNAVal which possesses an A residue in position 27 was shown to inhibit the methylation of G27 in E. coli tRNAMet. The data obtained confirm the suggestion that tRNA methylases recognizes the tertiary structure of tRNAs. They show also that the recognition and the proper catalytic action are two autonomous processes and that the former at least in its first stage is rather unspecific.
为了进一步阐明tRNA甲基化酶与tRNA相互作用的机制，分别对一些单个tRNA以及成对的tRNA进行了甲基化反应。在tRNA过量的条件下，当反应混合物中同时存在两种底物时，tRNA分子中位置相似的核苷酸的甲基化速率不会相加。研究表明，在第29位含有m5c的酵母tRNASer对其他单个tRNA中C29的甲基化具有抑制作用。在第27位含有A残基的酵母tRNAVal被证明可抑制大肠杆菌tRNAMet中G27的甲基化。所得数据证实了tRNA甲基化酶识别tRNA三级结构这一观点。这些数据还表明，识别和适当的催化作用是两个独立的过程，并且前者至少在其第一阶段相当不具有特异性。
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