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诺维科夫细胞中低甲基化信使核糖核酸的细胞质定位

Cytoplasmic location of undermethylated messenger RNA in Novikoff cells.

作者信息

Kaehler M, Coward J, Rottman F

出版信息

Nucleic Acids Res. 1979 Mar;6(3):1161-75. doi: 10.1093/nar/6.3.1161.

DOI:10.1093/nar/6.3.1161
PMID:440972
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327761/
Abstract

Novikoff cells in culture were labeled with L-[methyl-3H]methionine and [U-14C]uridine in the presence of (a) TubHcy2, (b) AdoHcy, (c) homocysteine, (d) tubercidin, or (e) without any additions. Only in cultures labeled in the presence of TubHcy were undermethylated cap structures observed to represent a significant portion of [3H]methyl radioactivity. Novikoff cells in culture were then simultaneously labeled with L-[methyl-3H]methionine and [32P]orthophosphate in the presence or absence of TubHcy. Total cytoplasmic, polysomal and monosomal poly(A)-containing RNAs were analyzed. Both monosomal and polysomal mRNA fractions from TubHcy-treated cells contain partially methylated cap structures, suggesting that 2'-O-methylation of the nucleoside adjacent to the pyrophosphate linkage in caps is not required for transport, ribosomal binding or translation. Comparison of nuclear and cytoplasmic cap structures from normal and inhibited cultures indicate that an altered mRNA population is generated in the presence of TubHcy.

摘要

在培养的诺维克夫细胞中，在以下条件下用L-[甲基-³H]甲硫氨酸和[U-¹⁴C]尿苷进行标记：(a) TubHcy2、(b) 腺苷高半胱氨酸、(c) 同型半胱氨酸、(d) 杀结核菌素，或(e) 不添加任何物质。只有在存在TubHcy的标记培养物中，才观察到低甲基化帽结构占[³H]甲基放射性的很大一部分。然后，在有或没有TubHcy的情况下，用L-[甲基-³H]甲硫氨酸和[³²P]正磷酸盐同时标记培养的诺维克夫细胞。分析了总细胞质、多核糖体和单核糖体中含poly(A)的RNA。来自TubHcy处理细胞的单核糖体和多核糖体mRNA部分均含有部分甲基化的帽结构，这表明帽中与焦磷酸键相邻的核苷的2'-O-甲基化对于转运、核糖体结合或翻译不是必需的。正常培养和受抑制培养的细胞核和细胞质帽结构的比较表明，在存在TubHcy的情况下会产生改变的mRNA群体。

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本文引用的文献

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The effects of inhibitors of RNA and DNA synthesis on protein synthesis and polysome levels in mouse L-cells.RNA和DNA合成抑制剂对小鼠L细胞中蛋白质合成及多核糖体水平的影响。
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Identification of methylated nucleosides in messenger RNA from Novikoff hepatoma cells.诺维科夫肝癌细胞信使核糖核酸中甲基化核苷的鉴定
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Cell. 1976 Dec;9(4 PT 2):645-53. doi: 10.1016/0092-8674(76)90128-8.
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Kinetics of formation of 5' terminal caps in mRNA.信使核糖核酸中5'末端帽结构形成的动力学
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Methyl labeling of HeLa cell hnRNA: a comparison with mRNA.HeLa细胞核内不均一RNA的甲基化标记：与信使RNA的比较。
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