The 8-isoprostaglandins: evidence for eight compounds in human semen.

Evidence is presented for the existence of a group of 8-iso prostaglandins in human semen, comprising 8-iso PG E1, 8-iso PG E2, 8-iso PG F1 alpha, 8-iso PG F2 alpha and the four corresponding 19-hydroxy prostaglandins. The E and F compounds have been positively identified by comparison of their mass spectra and chromatographic properties with those of authentic standards. Preliminary measurements of levels of these compounds in pooled semen are presented.