关于离体灌注大鼠肝脏中肝细胞增殖的一些观察
Some observations on liver cell proliferation in the isolated perfused rat liver.
作者信息
Lloyd E A, Crozier N, Pamphlet G, Wells M, Saunders S J
出版信息
Br J Exp Pathol. 1974 Jun;55(3):251-59.
Experiments were designed to test the hypothesis that in an isolated perfused system a regenerating liver is able to stimulate a normal liver to increase DNA replication after cross-circulation for one hour. The high degree of purity of DNA extracted by the “phenol” technique is demonstrated and the suitability of this technique for rat livers emphasized. The effect of chemiluminescence on tritiated thymidine is presented. The results of experiments presented here, together with other evidence, indicate the improbability of a stimulus being able to induce increased DNA synthesis in a normal liver after an exposure of only one hour.
设计实验以检验以下假设
在一个离体灌注系统中，再生肝在交叉循环一小时后能够刺激正常肝增加DNA复制。文中展示了用“苯酚”技术提取的DNA的高纯度，并强调了该技术对大鼠肝脏的适用性。介绍了化学发光对氚标记胸腺嘧啶核苷的影响。此处呈现的实验结果以及其他证据表明，仅暴露一小时后，一种刺激物能够诱导正常肝中DNA合成增加的可能性不大。
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