从鸡败血支原体中部分纯化一种膜相关脱氧核糖核酸复合物。

Partial purification of a membrane-associated deoxyribonucleic acid complex from Mycoplasma gallisepticum.

作者信息

Maniloff J, Quinlan D C

出版信息

J Bacteriol. 1974 Oct;120(1):495-501. doi: 10.1128/jb.120.1.495-501.1974.

DOI:10.1128/jb.120.1.495-501.1974

PMID:4420960

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC245788/

Abstract

A Mycoplasma gallisepticum subcellular fraction (P2), which contains the deoxyribonucleic acid replication complex, can be isolated by differential centrifugation of freeze-thaw-lysed cells. The nascent deoxyribonucleic acid is released from P2 by Lubrol-WX, sodium dodecyl sulfate, Pronase, and deoxyribonuclease, but not by saponin, ribonuclease, phospholipase C, or high-frequency sonic treatment. Sonic treatment further fractionates the cell ghost and allows partial purification, on sucrose density gradients, of a deoxyribonucleic acid replication complex attached to the cells' polar membrane-bleb-infrableb structures.

摘要

一种含有脱氧核糖核酸复制复合体的鸡毒支原体亚细胞组分（P2），可通过对冻融裂解细胞进行差速离心分离得到。新生的脱氧核糖核酸可被十二烷基硫酸钠月桂醇醚、十二烷基硫酸钠、链霉蛋白酶和脱氧核糖核酸酶从P2中释放出来，但不能被皂苷、核糖核酸酶、磷脂酶C或高频超声处理释放。超声处理进一步分离细胞空壳，并允许在蔗糖密度梯度上对附着于细胞极性膜泡-内陷泡结构的脱氧核糖核酸复制复合体进行部分纯化。