Quinlan D C, Maniloff J
J Bacteriol. 1973 Jul;115(1):117-20. doi: 10.1128/jb.115.1.117-120.1973.
Early log-phase cells of Mycoplasma gallisepticum A5969 were synchronized by holding in Eagle minimal essential medium (MEM) for 2 h. When transferred out of MEM into tryptose medium, the cells exhibited synchronous growth. Deoxyribonucleic acid (DNA) synthesis proceeded continuously during this growth but stopped during the period of cell division. One round of DNA replication was observed per cell doubling, and a unique region of DNA was found to be permanently bound to the membrane.
鸡败血支原体A5969的对数早期细胞通过在伊格尔基础培养基(MEM)中孵育2小时来实现同步化。当从MEM转移到胰蛋白胨培养基中时,细胞呈现出同步生长。在此生长过程中,脱氧核糖核酸(DNA)合成持续进行,但在细胞分裂期间停止。每细胞倍增观察到一轮DNA复制,并且发现一个独特的DNA区域永久结合在膜上。