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家兔嗅球在体时标记去甲肾上腺素的自发及神经诱发释放

Spontaneous and neurally evoked release of labelled noradrenaline from rabbit olfactory bulbs in vivo.

作者信息

Brenells A B

出版信息

J Physiol. 1974 Jul;240(2):279-93. doi: 10.1113/jphysiol.1974.sp010610.

DOI:10.1113/jphysiol.1974.sp010610
PMID:4424430
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1331016/
Abstract
  1. Rabbit olfactory bulbs were labelled with either [(3)H]noradrenaline (NA) and [(14)C]urea, or [(14)C]NA and [(3)H]inulin. The labelled substances released were collected by a modified cortical cup technique and estimated by liquid scintillation spectrometry.2. In many experiments only total radioactivity originating from labelled NA was measured. In such cases the term NA radioactivity is used.3. The spontaneous release of both NA radioactivity and marker radioactivity followed a multiphasic course. After 140 min the rate of efflux of NA radioactivity was significantly slower than that of the labelled marker.4. Stimulation of one medial olfactory tract, one lateral olfactory tract or the surface of one bulb resulted in a selective increase in release of NA radioactivity. The size of the increase was dependent on the intensity, frequency and duration of stimulation.5. There was a characteristic delay in the increased release of NA radioactivity following electrical stimulation. Acute lesioning of the olfactory tracts caudal to the stimulating electrode not only abolished this delay, but also resulted in a larger increase in release following stimulation.6. The radioactivity attributable to unchanged NA was initially 91%, but decreased to 66% after 4(1/2) hr of sample collection. Stimulation did not affect significantly the relative amounts of NA and metabolites released.7. Replacement of calcium in the Krebs solution by magnesium did not affect measurably the spontaneous release of NA radioactivity. However, after 1 hr; stimulation failed to increase the efflux of NA radioactivity.8. It is suggested that the results provide further evidence for a role for NA in synaptic transmission in the olfactory bulbs.
摘要

  1. 兔嗅球用[³H]去甲肾上腺素（NA）和[¹⁴C]尿素，或[¹⁴C]NA和[³H]菊粉进行标记。释放的标记物质通过改良的皮质杯技术收集，并通过液体闪烁光谱法进行估算。

  2. 在许多实验中，仅测量源自标记NA的总放射性。在这种情况下，使用术语NA放射性。

  3. NA放射性和标记物放射性的自发释放均呈多相过程。140分钟后，NA放射性的流出速率明显慢于标记物的流出速率。

  4. 刺激一侧内侧嗅束、一侧外侧嗅束或一个嗅球表面会导致NA放射性释放选择性增加。增加的幅度取决于刺激的强度、频率和持续时间。

  5. 电刺激后，NA放射性释放增加存在特征性延迟。刺激电极尾侧的嗅束急性损伤不仅消除了这种延迟，而且还导致刺激后释放增加幅度更大。

  6. 归因于未变化NA的放射性最初为91%，但在样品收集4.5小时后降至66%。刺激并未显著影响释放的NA和代谢产物的相对量。

  7. 用镁替代Krebs溶液中的钙对NA放射性的自发释放没有明显影响。然而，1小时后，刺激未能增加NA放射性的流出。

  8. 结果表明，这些结果为NA在嗅球突触传递中的作用提供了进一步的证据。

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本文引用的文献

1
The spontaneous and evoked release of acetylcholine from the cerebral cortex.乙酰胆碱从大脑皮层的自发释放和诱发释放。
J Physiol. 1963 Jan;165(1):98-116. doi: 10.1113/jphysiol.1963.sp007045.
2
ADRENERGIC MECHANISMS IN RABBIT OLFACTORY BULB.家兔嗅球中的肾上腺素能机制
Am J Physiol. 1964 Dec;207:1417-24. doi: 10.1152/ajplegacy.1964.207.6.1417.
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ANALYSIS OF INDIVIDUAL RABBIT OLFACTORY BULB NEURON RESPONSES TO THE MICROELECTROPHORESIS OF ACETYLCHOLINE, NOREPINEPHRINE AND SEROTONIN SYNERGISTS AND ANTAGONISTS.对单个兔嗅球神经元对乙酰胆碱、去甲肾上腺素和血清素协同剂及拮抗剂微电泳反应的分析。
J Pharmacol Exp Ther. 1964 Oct;146:16-23.
4
THE UPTAKE OF NORADRENALINE BY THE ISOLATED PERFUSED RAT HEART.去甲肾上腺素在离体灌注大鼠心脏中的摄取
Br J Pharmacol Chemother. 1963 Dec;21(3):523-37. doi: 10.1111/j.1476-5381.1963.tb02020.x.
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The effect of drugs on the release of norepinephrine-H from central nervous system tissued by electrical stimulation in vitro.药物对体外电刺激中枢神经系统组织中去甲肾上腺素 - H释放的影响。
J Pharmacol Exp Ther. 1967 Apr;156(1):31-8.
6
Extracellular space of rat cerebral cortex.大鼠大脑皮层的细胞外空间
Am J Physiol. 1967 Feb;212(2):367-70. doi: 10.1152/ajplegacy.1967.212.2.367.
7
Uptake of norepinephrine in periglomerular cells of the olfactory bulb of the mouse.去甲肾上腺素在小鼠嗅球球周细胞中的摄取。
Nature. 1966 May 28;210(5039):955-6. doi: 10.1038/210955a0.
8
Regional studies of catecholamines in the rat brain. I. The disposition of [3H]norepinephrine, [3H]dopamine and [3H]dopa in various regions of the brain.大鼠脑中儿茶酚胺的区域研究。I. [3H]去甲肾上腺素、[3H]多巴胺和[3H]多巴在脑不同区域的分布。
J Neurochem. 1966 Aug;13(8):655-69. doi: 10.1111/j.1471-4159.1966.tb09873.x.
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On the distribution and possible function of monamine nerve terminals in the olfactory bulb of the rabbit.关于兔嗅球中单胺神经末梢的分布及可能功能
Life Sci. 1965 Nov;4(21):2071-4. doi: 10.1016/0024-3205(65)90324-3.
10
Effect of environmental temperature on the turnover of noradrenaline in hypothalamus and other areas of rat brain.环境温度对大鼠下丘脑及其他脑区去甲肾上腺素周转的影响。
J Physiol. 1969 Jul;203(1):199-210. doi: 10.1113/jphysiol.1969.sp008859.