Huff E, Cole R M, Theodore T S
J Bacteriol. 1974 Oct;120(1):273-81. doi: 10.1128/jb.120.1.273-281.1974.
Mesosomal vesicles and plasma membranes of Staphylococcus aureus ATCC 6538P have been prepared and examined for the presence of lipoteichoic acid. Lipids were first removed by treatment with pyridine-acetic acid-butanol (22:31:100, vol/vol/vol) and chloroform-methanol (2:1, vol/vol). Subsequently, lipoteichoic acid was removed with 40% phenol in water. The lipoteichoic acid from mesosomal vesicles was characterized by (i) equimolar glycerol and phosphate, (ii) alanine upon hydrolysis (2 N NH(4)OH, 18 h, 22 C), and (iii) fatty acids, diglycerol triphosphate, glycerol monophosphate, and glycerol diphosphate upon alkaline hydrolysis (1 N NaOH, 3h, 100 C). The plasma membranes contained no lipoteichoic acid. The presence in mesosomal vesicles of 18% of the dry weight as lipoteichoic acid and its absence from plasma membranes provide the first major chemical differences between these organelles. A study of the lipoteichoic acid content in various fractions of the cell showed that the mesosomal vesicles were the major and probably the sole site for the localization of the lipoteichoic acid in these organisms. A new method for the preparation of mesosomes in increased yields is reported. A theory for the control of cell division involving lipoteichoic acid and the mesosome is proposed.
已制备金黄色葡萄球菌ATCC 6538P的间体囊泡和质膜，并检测其中脂磷壁酸的存在情况。首先用吡啶 - 乙酸 - 丁醇（22:31:100，体积/体积/体积）和氯仿 - 甲醇（2:1，体积/体积）处理以去除脂质。随后，用40%的苯酚水溶液去除脂磷壁酸。间体囊泡中的脂磷壁酸具有以下特征：（i）甘油和磷酸盐等摩尔；（ii）水解后产生丙氨酸（2N氢氧化铵，18小时，22℃）；（iii）碱性水解（1N氢氧化钠，3小时，100℃）后产生脂肪酸、二甘油三磷酸、甘油单磷酸和甘油二磷酸。质膜中不含脂磷壁酸。间体囊泡中脂磷壁酸占干重的18%，而质膜中没有，这是这些细胞器之间的第一个主要化学差异。对细胞不同组分中脂磷壁酸含量的研究表明，间体囊泡是这些生物体中脂磷壁酸定位的主要部位，可能也是唯一部位。报道了一种提高间体制备产量的新方法。提出了一种涉及脂磷壁酸和间体的细胞分裂控制理论。
