Wicken A J, Gibbens J W, Knox K W
J Bacteriol. 1973 Jan;113(1):365-72. doi: 10.1128/jb.113.1.365-372.1973.
Preparations of membrane lipoteichoic acid containing different amounts of protein were isolated from intact organisms of Lactobacillus fermenti NCTC 6991 by various procedures chosen for their ability to disrupt the hydrophobic interaction of lipoteichoic acid with other membrane components. The highest yield of lipoteichoic acid was obtained with hot aqueous phenol, and this preparation contained very little protein. Partial removal of cell lipids with chloroform-methanol followed by extraction with water at 100 C gave a lipoteichoic acid-protein complex that was a very effective immunogen; immunogenicity was shown to relate to protein content, though the specificity of the antibodies was directed against the teichoic acid component.
通过多种旨在破坏脂磷壁酸与其他膜成分疏水相互作用的程序，从发酵乳杆菌NCTC 6991的完整菌体中分离出含有不同蛋白质含量的膜脂磷壁酸制剂。用水相热苯酚法获得了最高产量的脂磷壁酸，该制剂含有的蛋白质极少。先用氯仿 - 甲醇部分去除细胞脂质，然后在100℃用水萃取，得到一种脂磷壁酸 - 蛋白质复合物，它是一种非常有效的免疫原；免疫原性与蛋白质含量有关，尽管抗体的特异性针对磷壁酸成分。
