通过区带离心机分离外周血中的异常细胞。
Separation of abnormal cells in the peripheral blood by means of the zonal centrifuge.
作者信息
Egan P M, Garrett J V, Gilbert C W
出版信息
J Clin Pathol. 1974 Sep;27(9):741-50. doi: 10.1136/jcp.27.9.741.
Abstract
The basic principles of rate centrifugation in the large volume zonal centrifuge and its application to the separation of abnormal or rarely occurring cells in the blood are described. Details of the method, gradient media, and collection and examination of the fractions obtained are given. A description of the cells found in normal human blood, in a subject undergoing known immunological stimulus, and in glandular fever and Hodgkin's disease is given and the implications are discussed.
摘要
本文描述了大容量区带离心机速率离心的基本原理及其在分离血液中异常或罕见细胞方面的应用。文中给出了该方法、梯度介质以及所得组分的收集和检测的详细信息。还给出了在正常人体血液、接受已知免疫刺激的受试者、腺热和霍奇金病中发现的细胞的描述，并对其意义进行了讨论。
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