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通过区带离心机分离外周血中的异常细胞。

Separation of abnormal cells in the peripheral blood by means of the zonal centrifuge.

作者信息

Egan P M, Garrett J V, Gilbert C W

出版信息

J Clin Pathol. 1974 Sep;27(9):741-50. doi: 10.1136/jcp.27.9.741.

DOI:10.1136/jcp.27.9.741
PMID:4426983
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC475461/
Abstract

The basic principles of rate centrifugation in the large volume zonal centrifuge and its application to the separation of abnormal or rarely occurring cells in the blood are described. Details of the method, gradient media, and collection and examination of the fractions obtained are given. A description of the cells found in normal human blood, in a subject undergoing known immunological stimulus, and in glandular fever and Hodgkin's disease is given and the implications are discussed.

摘要

本文描述了大容量区带离心机速率离心的基本原理及其在分离血液中异常或罕见细胞方面的应用。文中给出了该方法、梯度介质以及所得组分的收集和检测的详细信息。还给出了在正常人体血液、接受已知免疫刺激的受试者、腺热和霍奇金病中发现的细胞的描述，并对其意义进行了讨论。

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相似文献

1
Separation of abnormal cells in the peripheral blood by means of the zonal centrifuge.通过区带离心机分离外周血中的异常细胞。
J Clin Pathol. 1974 Sep;27(9):741-50. doi: 10.1136/jcp.27.9.741.
2
Abnormal cells in ther peripheral blood of patients with Hodgkin's disease. I Observation with light microscopy.霍奇金病患者外周血中的异常细胞。I. 光镜观察
Br J Haematol. 1974 Nov;28(3):317-22. doi: 10.1111/j.1365-2141.1974.tb00812.x.
3
The separation of human palatine tonsillar cells in the reorienting gradient zonal rotor: a large-capacity enrichment of plasma cells.在重定向梯度区带转子中分离人腭扁桃体细胞：高效富集浆细胞。
Clin Exp Immunol. 1980 Nov;42(2):370-7.
4
Increased proliferation of T lymphocytes in the blood of patients with Hodgkin's disease.霍奇金病患者血液中T淋巴细胞增殖增加。
Clin Exp Immunol. 1975 Jul;21(1):47-53.
5
Large-capacity separation of malignant cells and lymphocytes from the Furth mast cell tumor in a reorienting zonal rotor.在重新定向的区带转子中从弗思肥大细胞瘤中大规模分离恶性细胞和淋巴细胞。
Cancer Res. 1980 Jun;40(6):1791-6.
6
Significance of the changes in the circulating lymphoid cells in Hodgkin's disease.霍奇金病中循环淋巴细胞变化的意义。
Br Med J. 1969 May 24;2(5655):473-7. doi: 10.1136/bmj.2.5655.473.
7
Observations on abnormal cells in the peripheral blood and spleen in Hodgkin's disease.霍奇金病外周血及脾脏中异常细胞的观察
Br Med J. 1972 Jun 10;2(5814):609-11. doi: 10.1136/bmj.2.5814.609.
8
Isolation of lymphocyte populations.淋巴细胞群体的分离
Natl Cancer Inst Monogr. 1978 Dec(49):79-84.
9
A method for the separation of lymphocytes and plasma cells from the human palatine tonsil using sedimentation in an isokinetic gradient of Ficoll in tissue culture medium.一种在组织培养基中利用Ficoll等速梯度沉降从人腭扁桃体分离淋巴细胞和浆细胞的方法。
Immunology. 1975 Jan;28(1):161-70.
10
Human peripheral blood and bone marrow cell separation using density gradient centrifugation on Lymphoprep and Percoll in haematological diseases.
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本文引用的文献

1
Characterization of macromolecules by constant velocity sedimentation.通过等速沉降法对大分子进行表征。
Nature. 1967 Jul 22;215(5099):360-3. doi: 10.1038/215360a0.
2
Response of human blood lymphocytes to tuberculin PPD in tissue culture.人血淋巴细胞在组织培养中对结核菌素纯蛋白衍生物的反应。
Immunology. 1967 Apr;12(4):417-29.
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Sternberg-Reed cells in the peripheral blood of patients with Hodgkin's disease.霍奇金病患者外周血中的斯腾伯格-里德细胞。
Blood. 1966 Apr;27(4):544-56.
4
Significance of the changes in the circulating lymphoid cells in Hodgkin's disease.霍奇金病中循环淋巴细胞变化的意义。
Br Med J. 1969 May 24;2(5655):473-7. doi: 10.1136/bmj.2.5655.473.
5
The separation of different cell classes from lymphoid organs. II. The purification and analysis of lymphocyte populations by equilibrium density gradient centrifugation.从淋巴器官中分离不同细胞类别。II. 通过平衡密度梯度离心法对淋巴细胞群体进行纯化与分析。
Aust J Exp Biol Med Sci. 1968 Aug;46(4):375-96. doi: 10.1038/icb.1968.32.
6
The resolution of mixtures of viable mammalian cells into homogeneous fractions by zonal centrifugation.通过区带离心将活的哺乳动物细胞混合物分离成均匀的组分。
J Cell Biol. 1968 Feb;36(2):369-78. doi: 10.1083/jcb.36.2.369.
7
Estimation of experimental conditions that permit cell separations by velocity sedimentation on isokinetic gradients of Ficoll in tissue culture medium.在组织培养基中菲可立（Ficoll）等速梯度上通过速度沉降进行细胞分离的实验条件评估。
Anal Biochem. 1971 May;41(1):248-55. doi: 10.1016/0003-2697(71)90207-7.
8
Homogeneity of antibody-producing cells as analysed by their buoyant density in gradients of Ficoll.通过抗体产生细胞在Ficoll梯度中的浮力密度分析其同质性。
Immunology. 1970 Nov;19(5):817-29.
9
Tissue typing using a routine one-step lymphocyte separation procedure.采用常规的一步淋巴细胞分离程序进行组织分型。
Br J Haematol. 1970 Feb;18(2):229-35. doi: 10.1111/j.1365-2141.1970.tb01436.x.
10
Separation of mammalian cells using programmed gradient sedimentation.使用程序梯度沉降法分离哺乳动物细胞。
Exp Mol Pathol. 1969 Oct;11(2):139-52. doi: 10.1016/0014-4800(69)90003-3.