Coulson A S, Chalmers D G
Immunology. 1967 Apr;12(4):417-29.
Human lymphocyte suspensions containing more than 99.2 per cent small lymphocytes were prepared from peripheral blood using methyl cellulose and carbonyl iron. The human donors were skin tested with tuberculin PPD or tuberculin OT. Their lymphocytes were cultured in the presence of tuberculin PPD and the transformed cells which appeared in the cultures were accurately quantitated. When the corrected indices of response of lymphocyte cultures prepared from sensitized donors were plotted against the increasing amounts of tuberculin PPD used as a stimulus, a dose—response curve was obtained. The dose which gave the greatest distinction between lymphocytes from sensitized and from non-sensitized donors was 250 OT units of tuberculin PPD per ml of culture containing 2 × 10 small lymphocytes per ml. Analysis of the culture population at 20-hour intervals for 100 hours showed that the transformed cells seen were derived from small lymphocytes. Study of the behaviour of the stimulated cultures indicated that a 90-hour culture period was the most satisfactory for the accurate assessment of the immunological response. A correlation was established between the immunological status of the lymphocyte donor and the corrected index of response of his lymphocytes . Cultures from non-sensitized donors had a corrected index of response close to zero. Corrected indices of response from sensitized donors ranged from 2 to 94 depending on the sensitivity of the donor to tuberculin PPD.
使用甲基纤维素和羰基铁从外周血制备出小淋巴细胞含量超过99.2%的人淋巴细胞悬液。用人结核菌素纯蛋白衍生物（PPD）或旧结核菌素（OT）对人类供体进行皮肤试验。将他们的淋巴细胞在结核菌素PPD存在的情况下进行培养，并对培养物中出现的转化细胞进行精确计数。当将致敏供体制备的淋巴细胞培养物的校正反应指数与用作刺激物的结核菌素PPD用量增加情况作图时，得到了一条剂量-反应曲线。在每毫升含有2×10个小淋巴细胞的培养物中，能使致敏供体和未致敏供体的淋巴细胞产生最大差异的剂量是每毫升250个OT单位的结核菌素PPD。对培养群体每隔20小时进行100小时的分析表明，所见到的转化细胞来源于小淋巴细胞。对受刺激培养物行为的研究表明，90小时的培养期对于准确评估免疫反应最为合适。在淋巴细胞供体的免疫状态与其淋巴细胞的校正反应指数之间建立了相关性。未致敏供体的培养物校正反应指数接近零。致敏供体的校正反应指数范围为2至94，具体取决于供体对结核菌素PPD的敏感性。
