大鼠肝脏由3-羟基-3-甲基戊二酸生成乙酰乙酸以及所涉及的线粒体辅酶A转移酶活性的分离
Formation of acetoacetate from 3-hydroxy-3-methylglutarate by rat liver and isolation of a mitochondrial coenzyme A-transferase activity involved.
作者信息
Deana R, Meneghello R, Manzi L, Gregolin C
出版信息
Biochem J. 1974 Mar;138(3):481-6. doi: 10.1042/bj1380481.
Abstract
- Formation of acetoacetate from 3-hydroxy-3-methylglutarate was observed in the perfused rat liver. Production of 3.5mumol of acetoacetate/h per g of tissue was obtained. 2. Formation of acetoacetate was catalysed mainly by the mitochondrial fraction of the homogenized liver, at a rate of 62nmol/h per mg of protein. 3. Experiments with hydroxy-[3-(14)C]methylglutarate demonstrated that the acetoacetate formed was derived mainly from this compound. 4. A mitochondrial transferase activity catalysing the transfer of a CoA molecule from succinyl-CoA (3-carboxypropionyl-CoA) to hydroxymethylglutarate was shown. The K(m) value for hydroxymethylglutarate was 5x10(-3)m.
摘要
- 在灌注的大鼠肝脏中观察到了由3-羟基-3-甲基戊二酸形成乙酰乙酸的过程。每克组织每小时产生3.5微摩尔的乙酰乙酸。2. 乙酰乙酸的形成主要由匀浆肝脏的线粒体部分催化，速率为每毫克蛋白质每小时62纳摩尔。3. 用羟基-[3-(14)C]甲基戊二酸进行的实验表明，形成的乙酰乙酸主要来源于该化合物。4. 显示了一种线粒体转移酶活性，它催化辅酶A分子从琥珀酰辅酶A（3-羧基丙酰辅酶A）转移到羟甲基戊二酸。羟甲基戊二酸的K(m)值为5×10(-3)米。
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